TY - JOUR
T1 - Flow injection fluorometric determination of ascorbic acid using perylenebisimide-linked nitroxide
AU - Maki, Tomoharu
AU - Soh, Nobuaki
AU - Nakano, Koji
AU - Imato, Toshihiko
N1 - Funding Information:
This work was supported by a Grant-in-Aid for the Global COE Program, “Science for Future Molecular Systems” from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and Research Fellowships of the Japan Society for the Promotion of Science (JSPS) for Young Scientists.
PY - 2011/9/30
Y1 - 2011/9/30
N2 - A simple and sensitive flow injection fluorometric method for the determination of ascorbic acid is described. Perylenebisimide-linked nitroxide (PBILN) is used as a fluorescent reagent, which permits the selective determination of ascorbic acid. The fluorescence of the perylenebisimide moiety in PBILN is quenched by the nitroxide moiety, which is linked to the perylenebisimide. When a stream of a solution of ascorbic acid is merged with a stream of PBILN, the ascorbic acid reacts with the nitroxide moiety of PBILN to form hydroxylamine, and the fluorescence properties of the perylenebisimide moiety are recovered. As a result, a peak-shaped fluorescence signal is produced, which can be observed by a fluorescence detector located downstream. Under optimized conditions, a good linear relationship between the concentration of ascorbic acid and peak height in the concentration range from 0.5 to 10 μmol L-1 was found and the detection limit (S/N = 3) was 0.28 μmol L-1. The relative standard deviation for the determination of 4.0 μmol L-1 ascorbic acid samples was 1.0% (n = 5). The proposed method was applied to the determination of ascorbic acid in several soft drink beverages and the analytical results were in good agreement with those obtained using a conventional method.
AB - A simple and sensitive flow injection fluorometric method for the determination of ascorbic acid is described. Perylenebisimide-linked nitroxide (PBILN) is used as a fluorescent reagent, which permits the selective determination of ascorbic acid. The fluorescence of the perylenebisimide moiety in PBILN is quenched by the nitroxide moiety, which is linked to the perylenebisimide. When a stream of a solution of ascorbic acid is merged with a stream of PBILN, the ascorbic acid reacts with the nitroxide moiety of PBILN to form hydroxylamine, and the fluorescence properties of the perylenebisimide moiety are recovered. As a result, a peak-shaped fluorescence signal is produced, which can be observed by a fluorescence detector located downstream. Under optimized conditions, a good linear relationship between the concentration of ascorbic acid and peak height in the concentration range from 0.5 to 10 μmol L-1 was found and the detection limit (S/N = 3) was 0.28 μmol L-1. The relative standard deviation for the determination of 4.0 μmol L-1 ascorbic acid samples was 1.0% (n = 5). The proposed method was applied to the determination of ascorbic acid in several soft drink beverages and the analytical results were in good agreement with those obtained using a conventional method.
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U2 - 10.1016/j.talanta.2011.06.054
DO - 10.1016/j.talanta.2011.06.054
M3 - Article
C2 - 21872010
AN - SCOPUS:80052179356
SN - 0039-9140
VL - 85
SP - 1730
EP - 1733
JO - Talanta
JF - Talanta
IS - 4
ER -