FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Hiroshi Nonaka; Sho hei Fujishima; Sho hei Uchinomiya; Akio Ojida; Itaru Hamachi

doi:10.1016/j.bmcl.2009.09.122

FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Hiroshi Nonaka
, Sho hei Fujishima
, Sho hei Uchinomiya
, Akio Ojida
, Itaru Hamachi

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

Original language	English
Pages (from-to)	6696-6699
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	23
DOIs	https://doi.org/10.1016/j.bmcl.2009.09.122
Publication status	Published - Dec 1 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/j.bmcl.2009.09.122

Cite this

@article{1b8509773fb54aad924dffbc68646387,

title = "FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction",

abstract = "A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.",

author = "Hiroshi Nonaka and Fujishima, \{Sho hei\} and Uchinomiya, \{Sho hei\} and Akio Ojida and Itaru Hamachi",

year = "2009",

month = dec,

day = "1",

doi = "10.1016/j.bmcl.2009.09.122",

language = "English",

volume = "19",

pages = "6696--6699",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

number = "23",

}

TY - JOUR

T1 - FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

AU - Nonaka, Hiroshi

AU - Fujishima, Sho hei

AU - Uchinomiya, Sho hei

AU - Ojida, Akio

AU - Hamachi, Itaru

PY - 2009/12/1

Y1 - 2009/12/1

N2 - A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

AB - A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

UR - https://www.scopus.com/pages/publications/71849086452

UR - https://www.scopus.com/pages/publications/71849086452#tab=citedBy

U2 - 10.1016/j.bmcl.2009.09.122

DO - 10.1016/j.bmcl.2009.09.122

M3 - Article

C2 - 19837586

AN - SCOPUS:71849086452

SN - 0960-894X

VL - 19

SP - 6696

EP - 6699

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 23

ER -

FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this