FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Hiroshi Nonaka; Sho hei Fujishima; Sho hei Uchinomiya; Akio Ojida; Itaru Hamachi

doi:10.1016/j.bmcl.2009.09.122

FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Hiroshi Nonaka, Sho hei Fujishima, Sho hei Uchinomiya, Akio Ojida, Itaru Hamachi

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

Original language	English
Pages (from-to)	6696-6699
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	23
DOIs	https://doi.org/10.1016/j.bmcl.2009.09.122
Publication status	Published - Dec 1 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2009.09.122

Cite this

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title = "FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction",

abstract = "A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.",

author = "Hiroshi Nonaka and Fujishima, {Sho hei} and Uchinomiya, {Sho hei} and Akio Ojida and Itaru Hamachi",

year = "2009",

month = dec,

day = "1",

doi = "10.1016/j.bmcl.2009.09.122",

language = "English",

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journal = "Bioorganic and Medicinal Chemistry Letters",

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T1 - FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

AU - Nonaka, Hiroshi

AU - Fujishima, Sho hei

AU - Uchinomiya, Sho hei

AU - Ojida, Akio

AU - Hamachi, Itaru

PY - 2009/12/1

Y1 - 2009/12/1

N2 - A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

AB - A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

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DO - 10.1016/j.bmcl.2009.09.122

M3 - Article

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AN - SCOPUS:71849086452

SN - 0960-894X

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SP - 6696

EP - 6699

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 23

ER -

FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Abstract

All Science Journal Classification (ASJC) codes

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