TY - JOUR
T1 - First observation by fluorescence polarization of complexation between mRNA and the natural polysaccharide schizophyllan
AU - Karinaga, Ryouji
AU - Mizu, Masami
AU - Koumoto, Kazuya
AU - Anada, Takahisa
AU - Shinkai, Seiji
AU - Kimura, Taro
AU - Sakurai, Kazuo
PY - 2004/12/1
Y1 - 2004/12/1
N2 - Schizophyllan is a natural β-(1 → 3)-D-glucan that exists as a triple helix in H2O and as a single chain in dimethylsulfoxide (DMSO) or basic solution (pH >13). As we have already reported, when a homopolynucleotide (e.g., poly(dA), poly(A), or poly(C)) is added to a schizophyllan/DMSO solution, and, subsequently, DMSO is exchanged for H 2O, the single chain of schizophyllan forms a complex with the polynucleotide. Since eukaryotic mRNAs have poly(A) tails, we hypothesized that schizophyllan can bind to mRNA by interacting with this tail. However, we have not yet observed complexation between schizophyllan and mRNA after exchanging DMSO for H2O. In this report, we show that the complexation can be accelerated when the solution pHis changed from 13 to 7-8 in the presence of schizophyllan and polynucleotides. By this approach, we found that schizophyllan forms a complex with a yeast mRNA.
AB - Schizophyllan is a natural β-(1 → 3)-D-glucan that exists as a triple helix in H2O and as a single chain in dimethylsulfoxide (DMSO) or basic solution (pH >13). As we have already reported, when a homopolynucleotide (e.g., poly(dA), poly(A), or poly(C)) is added to a schizophyllan/DMSO solution, and, subsequently, DMSO is exchanged for H 2O, the single chain of schizophyllan forms a complex with the polynucleotide. Since eukaryotic mRNAs have poly(A) tails, we hypothesized that schizophyllan can bind to mRNA by interacting with this tail. However, we have not yet observed complexation between schizophyllan and mRNA after exchanging DMSO for H2O. In this report, we show that the complexation can be accelerated when the solution pHis changed from 13 to 7-8 in the presence of schizophyllan and polynucleotides. By this approach, we found that schizophyllan forms a complex with a yeast mRNA.
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U2 - 10.1002/cbdv.200490054
DO - 10.1002/cbdv.200490054
M3 - Article
C2 - 17191874
AN - SCOPUS:18144384008
SN - 1612-1872
VL - 1
SP - 634
EP - 639
JO - Chemistry and Biodiversity
JF - Chemistry and Biodiversity
IS - 4
ER -