TY - JOUR
T1 - Fibronectin adsorption on carbonate-containing hydroxyapatite
AU - Wang, Yunting
AU - Tsuru, Kanji
AU - Ishikawa, Kunio
AU - Yokoi, Taishi
AU - Kawashita, Masakazu
N1 - Funding Information:
This work was partially supported by the Naito Foundation Natural Science Scholarship. The authors thank Dr. Tomoyuki Ogawa (Graduate School of Engineering, Tohoku University) for the zeta potential measurements, Dr. Masanobu Kamitakahara (Graduate School of Environmental Studies, Tohoku University) for the SSA measurements, Mr. Kosei Kobayashi (Graduate School of Engineering, Tohoku University) for the elemental analysis, and Ms. Yoko Nakano (Graduate School of Engineering, Tohoku University) for the transmission electron microscopy and field-emission scanning electron microscopy observations.
Publisher Copyright:
© 2021 Elsevier Ltd and Techna Group S.r.l.
PY - 2021/4/15
Y1 - 2021/4/15
N2 - Carbonate-containing hydroxyapatite (CO3-HAp) shows higher osteoconductivity than pure hydroxyapatite but the underlying mechanism is unclear. As specific adsorption of proteins on CO3-HAp may affect cellular responses and osteoconductivity, we examined the contribution of protein adsorption to the high osteoconductivity observed with CO3-HAp. We prepared CO3-HAp powders with different carbonate contents (0.3, 4.8, or 8.3 wt%) and investigated the adsorption behaviour of fibronectin (Fn) on the powders. We found that the Fn adsorption capacity of CO3-HAp decreased with increasing carbonate content. Although the Hill or Langmuir isotherm was the best-fit for the adsorption of Fn onto CO3-HAp with a carbonate content of 0.3 or 4.8 wt%, the Brunauer–Emmett–Teller isotherm was the best-fit for Fn adsorption on CO3-HAp with an 8.3 wt% carbonate content. These results show that the carbonate content of CO3-HAp influences the adsorption behaviour of Fn and suggest that its specific adsorption contributes to the high osteoconductivity of CO3-HAp. The findings do not affect the clinical application of CO3-HAp directly, but they might lead to the development of bone substitutes with high osteoconductivity.
AB - Carbonate-containing hydroxyapatite (CO3-HAp) shows higher osteoconductivity than pure hydroxyapatite but the underlying mechanism is unclear. As specific adsorption of proteins on CO3-HAp may affect cellular responses and osteoconductivity, we examined the contribution of protein adsorption to the high osteoconductivity observed with CO3-HAp. We prepared CO3-HAp powders with different carbonate contents (0.3, 4.8, or 8.3 wt%) and investigated the adsorption behaviour of fibronectin (Fn) on the powders. We found that the Fn adsorption capacity of CO3-HAp decreased with increasing carbonate content. Although the Hill or Langmuir isotherm was the best-fit for the adsorption of Fn onto CO3-HAp with a carbonate content of 0.3 or 4.8 wt%, the Brunauer–Emmett–Teller isotherm was the best-fit for Fn adsorption on CO3-HAp with an 8.3 wt% carbonate content. These results show that the carbonate content of CO3-HAp influences the adsorption behaviour of Fn and suggest that its specific adsorption contributes to the high osteoconductivity of CO3-HAp. The findings do not affect the clinical application of CO3-HAp directly, but they might lead to the development of bone substitutes with high osteoconductivity.
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U2 - 10.1016/j.ceramint.2021.01.017
DO - 10.1016/j.ceramint.2021.01.017
M3 - Article
AN - SCOPUS:85099374031
SN - 0272-8842
VL - 47
SP - 11769
EP - 11776
JO - Ceramics International
JF - Ceramics International
IS - 8
ER -