TY - JOUR
T1 - Fetal hepatocyte-derived culture medium elicits adipocyte differentiation to bile duct cell lineages in a mouse model
AU - Ogawa, Hisataka
AU - Konno, Masamitsu
AU - Kawamoto, Koichi
AU - Nishida, Naohiro
AU - Koseki, Jun
AU - Mizushima, Tsunekazu
AU - Satoh, Taroh
AU - Eguchi, Hidetoshi
AU - Doki, Yuichiro
AU - Mori, Masaki
AU - Ishii, Hideshi
N1 - Funding Information:
The authors thank all the members of the laboratory for the discussion of the study and technical assistance. The present study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology and P-DIRECT; a Grant-in-Aid from the Ministry of Health, Labor and Welfare; a grant from the National Institute of Biomedical Innovation; and a grant from the Osaka University Drug Discovery Funds. Partial support was received from the Suzuken Memorial Foundation (M.K.), the Yasuda Medical Foundation (N.N.), the Pancreas Research Foundation (K.K.), the Nakatani Foundation (H.I.), and the Nakatomi Foundation of Japan (M.K.). Institutional endowments were received partially from Taiho Pharmaceutical Co., Ltd., Evidence Based Medical Research Center, Chugai Co., Ltd., Yakult Honsha Co., Ltd., and Merck Co., Ltd. These funding bodies had no role in the main experimental materials and methods, supplies or expenses, study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding Information:
The authors thank all the members of the laboratory for the discussion of the study and technical assistance. The present study was supported in part by a Grant‑in‑Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology and P‑DIRECT; a Grant‑in‑Aid from the Ministry of Health, Labor and Welfare; a grant from the National Institute of Biomedical Innovation; and a grant from the Osaka University Drug Discovery Funds. Partial support was received from the Suzuken Memorial Foundation (M.K.), the Yasuda Medical Foundation (N.N.), the Pancreas Research Foundation (K.K.), the Nakatani Foundation (H.I.), and the Nakatomi Foundation of Japan (M.K.). Institutional endowments were received partially from Taiho Pharmaceutical Co., Ltd., Evidence Based Medical Research Center, Chugai Co., Ltd., Yakult Honsha Co., Ltd., and Merck Co., Ltd. These funding bodies had no role in the main experimental materials and methods, supplies or expenses, study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2018, Spandidos Publications. All rights reserved.
PY - 2018/5
Y1 - 2018/5
N2 - Fetal cells in the developmental stages function with a distinct mechanism in comparison to adult tissues, which may be a useful source for regenerative medicine in postnatal medicine; however, the precise molecular mechanism remains to be elucidated fully. The present study investigated murine fetal hepatocytes, which were cultured in vitro, and the supernatants were used for the culture with murine adipose tissue-derived cells. Notably, the results indicated that fetal hepatocyte-derived culture medium elicits the induction of differentiation of adipose tissue-derived cells to bile duct cell lineages, but not to hepatocyte lineages in mice. This indicates that fetal cells possess the multi potentials, which are already absent in adults, and may be useful for regenerative medicine in future.
AB - Fetal cells in the developmental stages function with a distinct mechanism in comparison to adult tissues, which may be a useful source for regenerative medicine in postnatal medicine; however, the precise molecular mechanism remains to be elucidated fully. The present study investigated murine fetal hepatocytes, which were cultured in vitro, and the supernatants were used for the culture with murine adipose tissue-derived cells. Notably, the results indicated that fetal hepatocyte-derived culture medium elicits the induction of differentiation of adipose tissue-derived cells to bile duct cell lineages, but not to hepatocyte lineages in mice. This indicates that fetal cells possess the multi potentials, which are already absent in adults, and may be useful for regenerative medicine in future.
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U2 - 10.3892/br.2018.1080
DO - 10.3892/br.2018.1080
M3 - Article
AN - SCOPUS:85044632488
SN - 2049-9434
VL - 8
SP - 497
EP - 499
JO - Biomedical Reports
JF - Biomedical Reports
IS - 5
ER -