TY - JOUR
T1 - Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration
AU - Hirao-Suzuki, Masayo
AU - Koga, Takayuki
AU - Sakai, Genki
AU - Kobayashi, Takanobu
AU - Ishii, Yuji
AU - Miyazawa, Hiroshi
AU - Takiguchi, Masufumi
AU - Sugihara, Narumi
AU - Toda, Akihisa
AU - Ohara, Masahiro
AU - Takeda, Shuso
N1 - Funding Information:
This study was supported in part by the Grant-in-Aid for Scientific Research (C) [ 25460182 and 17K08402 (to S.T.)] from the Japan Society for the Promotion of Science (JSPS) KAKENHI and by the Setouchi Satoyama-Satoumi Research Project Grant of the Fukuyama University (to S.T. and N.S.).
Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2020/10/15
Y1 - 2020/10/15
N2 - The functional role of fatty acid 2-hydroxylase (FA2H) is controversial in the field of cancer biology due to the dual role of FA2H, particularly related to its interaction with triple-negative breast cancer (TNBC). A previous biochemical- and clinical-focused study suggested that FA2H could dampen TNBC aggressiveness. However, another epidemiological study demonstrated that FA2H expression is associated with shorter disease-free survival in TNBC cases. We reported that FA2H is a peroxisome proliferator-activated receptor α (PPARα)-regulated gene in human breast cancer MDA-MB-231 cells, in vitro experimental models for TNBC analysis. PPARα activation by its ligand reportedly results in an aggressive MDA-MB-231 cell phenotype, as well as estrogen receptor α (ERα)-positive MCF-7 cells. The results of this study show that i) MDA-MB-231 cells express very low levels of FA2H compared to the MCF-7 cells, reflecting a low basal-level PPARα-driven transcriptional activity compared to the MCF-7 cells, and ii) the increased FA2H expression stimulates the MDA-MB-231 and MCF-7 breast cancer cell migration without affecting proliferation. Taken together, our findings indicate that FA2H might be a breast cancer cell migration stimulator, independently of the ERα expression status.
AB - The functional role of fatty acid 2-hydroxylase (FA2H) is controversial in the field of cancer biology due to the dual role of FA2H, particularly related to its interaction with triple-negative breast cancer (TNBC). A previous biochemical- and clinical-focused study suggested that FA2H could dampen TNBC aggressiveness. However, another epidemiological study demonstrated that FA2H expression is associated with shorter disease-free survival in TNBC cases. We reported that FA2H is a peroxisome proliferator-activated receptor α (PPARα)-regulated gene in human breast cancer MDA-MB-231 cells, in vitro experimental models for TNBC analysis. PPARα activation by its ligand reportedly results in an aggressive MDA-MB-231 cell phenotype, as well as estrogen receptor α (ERα)-positive MCF-7 cells. The results of this study show that i) MDA-MB-231 cells express very low levels of FA2H compared to the MCF-7 cells, reflecting a low basal-level PPARα-driven transcriptional activity compared to the MCF-7 cells, and ii) the increased FA2H expression stimulates the MDA-MB-231 and MCF-7 breast cancer cell migration without affecting proliferation. Taken together, our findings indicate that FA2H might be a breast cancer cell migration stimulator, independently of the ERα expression status.
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U2 - 10.1016/j.bbrc.2020.07.043
DO - 10.1016/j.bbrc.2020.07.043
M3 - Article
C2 - 32798015
AN - SCOPUS:85089294065
SN - 0006-291X
VL - 531
SP - 215
EP - 222
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
