Fate redirection of hippocampal astrocytes toward neuronal lineage by aggregate culture

Makoto Yanagisawa, Kinichi Nakashima, Wataru Ochiai, Takumi Takizawa, Takao Setoguchi, Atsumi Uemura, Makiko Takizawa, Ikuo Nobuhisa, Tetsuya Taga

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Mammalian cells that have been committed to a certain cell lineage cannot be directed to other lineages. However, some astrocytes in the mammalian brains have been reported to represent plasticity to redirect to other cell lineages. We found that mouse hippocampal astrocytes cultured in aggregate forms of "astrosphere", redirected to MAP2-positive immature neurons. In astrospheres, basic HLH factors positively regulating neuronal differentiation were up-regulated and Id3 inhibiting basic HLH factors was down-regulated. Ectopic Id3 induction repressed redirection of astrocytes to a neuronal lineage, suggesting that astrosphere formation induced plasticity of astrocytes by changing the gene expression patterns.

Original languageEnglish
Pages (from-to)176-182
Number of pages7
JournalNeuroscience Research
Issue number2
Publication statusPublished - Oct 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Neuroscience


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