TY - JOUR
T1 - FANCD2 mRNA overexpression is a bona fide indicator of lymph node metastasis in human colorectal cancer
AU - Ozawa, Heita
AU - Iwatsuki, Masaaki
AU - Mimori, Koshi
AU - Sato, Tetsuya
AU - Johansson, Fredrik
AU - Toh, Hiroyuki
AU - Watanabe, Masahiko
AU - Mori, Masaki
N1 - Funding Information:
ACKNOWLEDGMENT We thank S. Waaijenborg for kindly providing the PCCA software. We also thank Dr. Yoshikawa, Dr. Ohishi, Ms. T Shimooka, Mrs. K Ogata, Ms. M Kasagi, Ms. Y Nakagawa, and MsT Kawano for their excellent advice and technical assistance. This work was supported in part by the following grants and foundations: Core Research for Evolutional Science and Technology, Grants-in-Aid for Scientific Research from the Ministry of Health, Labour and Welfare No. 17-15, and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, No. 20790960 and 19390336.
PY - 2010/9
Y1 - 2010/9
N2 - Background: Lymph node metastasis is widely accepted as one of the most important determinants of prognosis in colorectal cancer (CRC) patients. Therefore, there is an urgent need to identify molecular markers that can be used to predict lymph node metastasis. Materials and Methods: Candidate genes were found using LMD and cDNA microarrays in a large-scale study of CRC, followed by Penalized Canonical Correlation Analysis (PCCA). We focused on the Fanconi anemia, complementation group D2 (FANCD2) gene and evaluated FANCD2 mRNA expression in 133 CRC cases to determine the clinicopathological significance of FANCD2 expression. Results: The mean level of FANCD2 mRNA expression in tumor tissue specimens was significantly higher than in nontumor tissue. FANCD2 expression was found to be a significant factor affecting lymph node metastasis: the high FANCD2 expression group had a significantly poorer prognosis than the low expression group. Conclusions: This study suggests that PCCA can be used to identify genes related to clinicopathological features. Furthermore, high FANCD2 expression was a significant independent factor for lymph node metastasis.
AB - Background: Lymph node metastasis is widely accepted as one of the most important determinants of prognosis in colorectal cancer (CRC) patients. Therefore, there is an urgent need to identify molecular markers that can be used to predict lymph node metastasis. Materials and Methods: Candidate genes were found using LMD and cDNA microarrays in a large-scale study of CRC, followed by Penalized Canonical Correlation Analysis (PCCA). We focused on the Fanconi anemia, complementation group D2 (FANCD2) gene and evaluated FANCD2 mRNA expression in 133 CRC cases to determine the clinicopathological significance of FANCD2 expression. Results: The mean level of FANCD2 mRNA expression in tumor tissue specimens was significantly higher than in nontumor tissue. FANCD2 expression was found to be a significant factor affecting lymph node metastasis: the high FANCD2 expression group had a significantly poorer prognosis than the low expression group. Conclusions: This study suggests that PCCA can be used to identify genes related to clinicopathological features. Furthermore, high FANCD2 expression was a significant independent factor for lymph node metastasis.
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U2 - 10.1245/s10434-010-1002-7
DO - 10.1245/s10434-010-1002-7
M3 - Article
C2 - 20339950
AN - SCOPUS:77956343107
SN - 1068-9265
VL - 17
SP - 2341
EP - 2348
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
IS - 9
ER -
