Failure of t cell receptor negative selection in murine intestinal intra-epitheliallymphocytes

Shinji Murosaki, Yasunobu Yoshikai, Atsunori Ishida, Takao Nakamura, Goro Matsuzaki, Hiroaki Taki Moto, Hiroyuki Yuuki, Kikuo Nomoto

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60 Citations (Scopus)


The intestinal intra-epithelial lymphocytes (IEL) are divided into several subsets on the basis of expression of T cell receptor (TCR) a/3 and y6, intensity of Thy-1 expression and expression ofLyt-3 chain. To investigate the differentiation pathway of the IEL, we examined the repertoire of\f0 segments of T cells in the IEL in BALB/c (H-2d, Mls-1b2a) or AKR/J (H-2k, Mls-1a2b) mice. Among freshly isolated IEL, an appreciable number of T cells bearing Vβ3 or Vβ11, which recognize Mls-2a- or MHC IE-encoded molecules respectively, were detected in BALB/c mice.Similarly, in AKR/J mice, IEL contained appreciable levels of V^6-bearing T cells. Vβ3- orVβ11 -bearing T cells in the IEL in BALB/c mice increased to a significant level when incubatedwith staphylococcal enterotoxin A which specifically stimulates V03- and Vg11 -bearing T cells.Most of IEL without clonal deletion expressed Lyt-2 but not Lyt-3 antigens. Such T ceils werehardly detected in other organs, including liver. Our results indicate that TCRa/3-bearing intestinalIEL that have not undergone negative selection may have differentiated outside the thymus, presumably at a local site of the intestine and can respond normally to the signal via their TCR.

Original languageEnglish
Pages (from-to)1005-1013
Number of pages9
JournalInternational immunology
Issue number10
Publication statusPublished - Oct 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology


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