Facilitation of dissociation reaction of nucleotides bound to Mycobacterium tuberculosis DnaA

Kohji Yamamoto, Meredith Moomey, Malini Rajagopalan, Murty V.V.S. Madiraju

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3 Citations (Scopus)


Acidic phospholipids have been shown to promote dissociation of bound nucleotides from Mycobacterium tuberculosis DnaA (DnaATB) purified under denaturing conditions [Yamamoto et al., (2002) Modulation of Mycobacterium tuberculosis DnaA protein-adenine-nucleotide interactions by acidic phospholipids. Biochem. J., 363, 305-311]. In the present study, we show that a majority of DnaATB in non-overproducing cells of M. tuberculosis is membrane associated. Estimation of phospholipid phosphorus following chloroform: methanol extraction of soluble DnaATB purified under native conditions (nDnaATB) confirmed the association with phospholipids. nDnaATB exhibited weak ATPase activity, and rapidly exchanged ATP for bound ADP in the absence of any added phospholipids. We suggest that the outcome of intra-cellular DnaATB-nucleotide interactions, hence DnaATB activity, is influenced by phospholipids.

Original languageEnglish
Pages (from-to)759-764
Number of pages6
JournalJournal of biochemistry
Issue number6
Publication statusPublished - Jun 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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