TY - JOUR
T1 - Facile method for the preparation of lyso-GM1 and lyso-GM2
AU - Ando, Takayuki
AU - Li, Su Chen
AU - Ito, Makoto
AU - Li, Yu Teh
N1 - Funding Information:
This work was supported by NIH Grant NS 09626 (Y.-T.L.) and grant-in aid for Scientific Research on Priority Area 1240204 from the Ministry of Education, Culture, Sport, Science and Technology, Japan (M.I.).
PY - 2005/6/17
Y1 - 2005/6/17
N2 - This paper reports a facile method for the preparation of lyso-GM1 [Galβ1 → 3GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-sphingosine] and lyso-GM2 [GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-sphingosine], respectively, from GM1 [Galβ1 → 3GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-Cer] and GM2 [GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-Cer], using sphingolipid ceramide deacylase and high performance anion-exchange chromatography (HPAEC). The enzymatically released lyso-GM1 and/or lyso-GM2 was effectively separated from its parent ganglioside by HPAEC using a Mono Q HR 5/5 column with an Amersham Biosciences fast protein liquid chromatography system. The yield was almost quantitative and the separation completed in approximately 3 h. This method is more convenient and effective than the conventional method using alkaline hydrolysis and silicic acid chromatography to generate and purify lyso-gangliosides.
AB - This paper reports a facile method for the preparation of lyso-GM1 [Galβ1 → 3GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-sphingosine] and lyso-GM2 [GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-sphingosine], respectively, from GM1 [Galβ1 → 3GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-Cer] and GM2 [GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-Cer], using sphingolipid ceramide deacylase and high performance anion-exchange chromatography (HPAEC). The enzymatically released lyso-GM1 and/or lyso-GM2 was effectively separated from its parent ganglioside by HPAEC using a Mono Q HR 5/5 column with an Amersham Biosciences fast protein liquid chromatography system. The yield was almost quantitative and the separation completed in approximately 3 h. This method is more convenient and effective than the conventional method using alkaline hydrolysis and silicic acid chromatography to generate and purify lyso-gangliosides.
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U2 - 10.1016/j.chroma.2005.04.058
DO - 10.1016/j.chroma.2005.04.058
M3 - Article
C2 - 16010718
AN - SCOPUS:20444462383
SN - 0021-9673
VL - 1078
SP - 193
EP - 195
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -