TY - GEN
T1 - Fabrication of 3D tissue-like structure using magnetite nanoparticles and magnetic force
AU - Ito, Akira
AU - Ino, Kousuke
AU - Shimizu, Kazunori
AU - Honda, Hiroyuki
AU - Kamihira, Masamichi
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Novel technologies to establish 3D tissue-like constructs are desired for tissue engineering. In the present study, magnetic force and magnetite nanoparticles were used to construct a layered mesenchymal stem cell (MSC) sheet, a layered cardiomyocyte sheet, and a layered fibroblast sheet involving capillaries. Magnetite cationic liposomes (MCLs) were taken up by the target cells. When a magnet was set under a tissue culture dish, magnetically labeled target cells were attracted and then adhered to form a layered cell sheet. MSC sheets were harvested and transplanted into the bone defect in cranium of nude rats, resulted in formation of new bones surrounded by osteoblast-like cells. For cardiomyocyte sheets, the immunofluorescence staining revealed the existence of gap junctions within the cardiomyocyte sheets. For fibroblast sheets, normal human dermal fibroblasts (NHDFs) sheets contained the major dermal extracellular matrix components. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NIIDF sheets, resulted in tube-like formation of HUVECs. These results suggest that this novel use of magnetite nanoparticles and magnetic force, which we refer to as "magnetic force-based tissue engineering (Mag-TE)", offers a major advancement in tissue engineering.
AB - Novel technologies to establish 3D tissue-like constructs are desired for tissue engineering. In the present study, magnetic force and magnetite nanoparticles were used to construct a layered mesenchymal stem cell (MSC) sheet, a layered cardiomyocyte sheet, and a layered fibroblast sheet involving capillaries. Magnetite cationic liposomes (MCLs) were taken up by the target cells. When a magnet was set under a tissue culture dish, magnetically labeled target cells were attracted and then adhered to form a layered cell sheet. MSC sheets were harvested and transplanted into the bone defect in cranium of nude rats, resulted in formation of new bones surrounded by osteoblast-like cells. For cardiomyocyte sheets, the immunofluorescence staining revealed the existence of gap junctions within the cardiomyocyte sheets. For fibroblast sheets, normal human dermal fibroblasts (NHDFs) sheets contained the major dermal extracellular matrix components. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NIIDF sheets, resulted in tube-like formation of HUVECs. These results suggest that this novel use of magnetite nanoparticles and magnetic force, which we refer to as "magnetic force-based tissue engineering (Mag-TE)", offers a major advancement in tissue engineering.
UR - http://www.scopus.com/inward/record.url?scp=50449095451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50449095451&partnerID=8YFLogxK
U2 - 10.1109/MHS.2006.320291
DO - 10.1109/MHS.2006.320291
M3 - Conference contribution
AN - SCOPUS:50449095451
SN - 1424407176
SN - 9781424407170
T3 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
BT - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
T2 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Y2 - 5 November 2006 through 8 November 2006
ER -