TY - CHAP
T1 - Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars
AU - Kasama, Toshihiro
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
N1 - Publisher Copyright:
© Springer Science+Business Media LLC 2017.
PY - 2017
Y1 - 2017
N2 - Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.
AB - Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.
UR - https://www.scopus.com/pages/publications/85008452835
UR - https://www.scopus.com/pages/publications/85008452835#tab=citedBy
U2 - 10.1007/978-1-4939-6734-6_4
DO - 10.1007/978-1-4939-6734-6_4
M3 - Chapter
C2 - 28044286
AN - SCOPUS:85008452835
T3 - Methods in Molecular Biology
SP - 49
EP - 56
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -