Extractive alkylation and gas chromatographic analysis of sulfide

A sensitive analysis of sulfide in blood was established, using an extractive alkylation technique. Pentafluorobenzyl bromide was used as the alkylating agent, tetradecyldimethyl-benzylammonium chloride as the phase-transfer catalyst, and potassium dihydrogenphosphate as the buffer to suppress the formation of sulfide. Mass fragmentography was used to identify the sulfide derivative and gas chromatography with an electron capture detector was used for quantitative determination, with the lowest limit of detection being about 0.01 μg/g. The blood level of rats exposed to hydrogen sulfide was also determined.