TY - JOUR
T1 - Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells
AU - Hamano, Sayuri
AU - Tomokiyo, Atsushi
AU - Hasegawa, Daigaku
AU - Yoshida, Shinichiro
AU - Sugii, Hideki
AU - Mitarai, Hiromi
AU - Fujino, Shoko
AU - Wada, Naohisa
AU - Maeda, Hidefumi
N1 - Funding Information:
We thank Drs. Serita, Mizumachi, Sonoda, Nozu, Itoya-ma, Ono, and Taketomi-Saito for their great support in the preparation of this work. This work was supported by JSPS KAKENHI grant numbers JP15H05023, JP16K20457, JP16K20458, JP17H01598, and JP17H04385.
Publisher Copyright:
© 2018, Mary Ann Liebert, Inc.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/1/15
Y1 - 2018/1/15
N2 - The periodontal ligament (PDL) plays an important role in anchoring teeth in the bone socket. Damage to the PDL, such as after severe inflammation, can be treated with a therapeutic strategy that uses stem cells derived from PDL tissue (PDLSCs), a strategy that has received intense scrutiny over the past decade. However, there is an insufficient number of PDLSCs within the PDL for treating such damage. Therefore, we sought to induce the differentiation of induced pluripotent stem (iPS) cells into PDLSCs as an initial step toward PDL therapy. To this end, we first induced iPS cells into neural crest (NC)-like cells. We then captured the p75 neurotrophic receptor-positive cells (iPS-NC cells) and cultured them on an extracellular matrix (ECM) produced by human PDL cells (iPS-NC-PDL cells). These iPS-NC-PDL cells showed reduced expression of embryonic stem cell and NC cell markers as compared with iPS and iPS-NC cells, and enrichment of mesenchymal stem cell markers. The cells also had a higher proliferative capacity, multipotency, and elevated expression of PDL-related markers than iPS-NC cells cultured on fibronectin and laminin (iPS-NC-FL cells) or ECM produced by human skin fibroblast cells (iPS-NC-SF cells). Overall, we present a culture method to produce high number of PDLSC-like cells from iPS cells as a first step toward a strategy for PDL regeneration.
AB - The periodontal ligament (PDL) plays an important role in anchoring teeth in the bone socket. Damage to the PDL, such as after severe inflammation, can be treated with a therapeutic strategy that uses stem cells derived from PDL tissue (PDLSCs), a strategy that has received intense scrutiny over the past decade. However, there is an insufficient number of PDLSCs within the PDL for treating such damage. Therefore, we sought to induce the differentiation of induced pluripotent stem (iPS) cells into PDLSCs as an initial step toward PDL therapy. To this end, we first induced iPS cells into neural crest (NC)-like cells. We then captured the p75 neurotrophic receptor-positive cells (iPS-NC cells) and cultured them on an extracellular matrix (ECM) produced by human PDL cells (iPS-NC-PDL cells). These iPS-NC-PDL cells showed reduced expression of embryonic stem cell and NC cell markers as compared with iPS and iPS-NC cells, and enrichment of mesenchymal stem cell markers. The cells also had a higher proliferative capacity, multipotency, and elevated expression of PDL-related markers than iPS-NC cells cultured on fibronectin and laminin (iPS-NC-FL cells) or ECM produced by human skin fibroblast cells (iPS-NC-SF cells). Overall, we present a culture method to produce high number of PDLSC-like cells from iPS cells as a first step toward a strategy for PDL regeneration.
UR - http://www.scopus.com/inward/record.url?scp=85044622727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85044622727&partnerID=8YFLogxK
U2 - 10.1089/scd.2017.0077
DO - 10.1089/scd.2017.0077
M3 - Article
C2 - 29160151
AN - SCOPUS:85044622727
SN - 1547-3287
VL - 27
SP - 100
EP - 111
JO - Stem cells and development
JF - Stem cells and development
IS - 2
ER -