Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis

Yutaka Konya, Takeshi Bamba, Eiichiro Fukusaki

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.

Original languageEnglish
Pages (from-to)349-353
Number of pages5
JournalJournal of Bioscience and Bioengineering
Issue number3
Publication statusPublished - Mar 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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