TY - JOUR
T1 - Expression profiling of cumulus cells reveals functional changes during ovulation and central roles of prostaglandin EP2 receptor in cAMP signaling
AU - Tamba, Shigero
AU - Yodoi, Rieko
AU - Morimoto, Kazushi
AU - Inazumi, Tomoaki
AU - Sukeno, Mamiko
AU - Segi-Nishida, Eri
AU - Okuno, Yasushi
AU - Tsujimoto, Gozoh
AU - Narumiya, Shuh
AU - Sugimoto, Yukihiko
N1 - Funding Information:
S.T. was supported by a research fellowship from the Japan Society for the Promotion of Science for Young Scientists. This work was supported in part by Grants-in-Aid for Scientific Research and on Priority Areas ‘Applied Genomics’ and ‘Mechanisms of Sex Differentiation’ from the Ministry of Education, Culture, Sports, Science, and Technology of Japan , from the Ministry of Health and Labor of Japan , from the National Institute of Biomedical Innovation of Japan , from the Mochida Memorial Foundation and the Naito Foundation . We thank K. Nakayama and S. Tsuchiya for their continuous support. We also thank Y. Hirose, M. Hirose, and Y. Noda for their technical support and helpful advice. We are also grateful to H.A. Popiel and Y. Nakaminami for careful reading of the manuscript and secretary assistance, respectively.
PY - 2010/6
Y1 - 2010/6
N2 - To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2-/- cumuli before and after ovulation by using microarrays. We prepared cumulus cells from mice just before and 3, 9 and 14 h after human chorionic gonadotropin injection. Key genes including cAMP-related and epidermal growth factor (EGF) genes, as well as extracellular matrix- (ECM-) related and chemokine genes were up-regulated in WT cumuli at 3 h and 14 h, respectively. Ptger2 deficiency differently affected the expression of many of the key genes at 3 h and 14 h. These results indicate that the gene expression profile of cumulus cells greatly differs before and after ovulation, and in each situation, PGE2-EP2 signaling plays a critical role in cAMP-regulated gene expression in the cumulus cells under physiological conditions.
AB - To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2-/- cumuli before and after ovulation by using microarrays. We prepared cumulus cells from mice just before and 3, 9 and 14 h after human chorionic gonadotropin injection. Key genes including cAMP-related and epidermal growth factor (EGF) genes, as well as extracellular matrix- (ECM-) related and chemokine genes were up-regulated in WT cumuli at 3 h and 14 h, respectively. Ptger2 deficiency differently affected the expression of many of the key genes at 3 h and 14 h. These results indicate that the gene expression profile of cumulus cells greatly differs before and after ovulation, and in each situation, PGE2-EP2 signaling plays a critical role in cAMP-regulated gene expression in the cumulus cells under physiological conditions.
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U2 - 10.1016/j.biochi.2010.04.006
DO - 10.1016/j.biochi.2010.04.006
M3 - Article
C2 - 20399827
AN - SCOPUS:77953285229
SN - 0300-9084
VL - 92
SP - 665
EP - 675
JO - Biochimie
JF - Biochimie
IS - 6
ER -