TY - JOUR
T1 - Expression profiles of genes associated with viral entry in HCV-infected human liver
AU - Nakamuta, M.
AU - Fujino, T.
AU - Yada, R.
AU - Aoyagi, Y.
AU - Yasutake, K.
AU - Kohjima, M.
AU - Fukuizumi, K.
AU - Yoshimoto, T.
AU - Harada, N.
AU - Yada, M.
AU - Kato, M.
AU - Kotoh, K.
AU - Taketomi, A.
AU - Maehara, Y.
AU - Nakashima, M.
AU - Enjoji, M.
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Recent studies have demonstrated that several cellular factors are involved in entry of hepatitis C virus (HCV) into host cells. Detailed gene expression profiles of these factors in HCV-infected livers have not been reported for humans. Transcriptional levels of LDL receptor (LDLR), CD81, scavenger receptor class B type I (SR-BI), claudin-1, and occludin genes in liver samples from patients with chronic hepatitis C were investigated. Serum levels of LDL-cholesterol (LDL-C) and HCV core antigen were also evaluated, and expression of claudin-1 and occludin were immunohistochemically analyzed. Compared with normal liver, transcription of LDLR and claudin-1 genes was significantly suppressed (P<0.0001) and occludin transcription was significantly up-regulated in HCV-infected livers (P<0.0001). Significant positive correlations were found for LDLR versus occludin, LDLR versus claudin-1, occludin versus claudin-1, and CD81 versus SR-BI in HCV-infected (P=0.0012, P<0.0001, P=0.0004, and P<0.0001, respectively) and normal livers (P<0.0001, P=0.0051, P<0.0001, and P<0.0001, respectively). Positive correlation was observed between serum levels of HCV core antigen and LDL-C (P=0.0147), with their levels negatively correlated to LDLR (P=0.0270 and P=0.0021, respectively). Immunohistochemically, hepatocellular expression of claudin-1 and occludin was increased in HCV-infected livers. Different levels of expression were demonstrated at the mRNA and protein levels for occludin and claudin-1 in HCV-infected and normal livers. Correlation of elements associated with viral entry was comparable in HCV-infected and normal livers.
AB - Recent studies have demonstrated that several cellular factors are involved in entry of hepatitis C virus (HCV) into host cells. Detailed gene expression profiles of these factors in HCV-infected livers have not been reported for humans. Transcriptional levels of LDL receptor (LDLR), CD81, scavenger receptor class B type I (SR-BI), claudin-1, and occludin genes in liver samples from patients with chronic hepatitis C were investigated. Serum levels of LDL-cholesterol (LDL-C) and HCV core antigen were also evaluated, and expression of claudin-1 and occludin were immunohistochemically analyzed. Compared with normal liver, transcription of LDLR and claudin-1 genes was significantly suppressed (P<0.0001) and occludin transcription was significantly up-regulated in HCV-infected livers (P<0.0001). Significant positive correlations were found for LDLR versus occludin, LDLR versus claudin-1, occludin versus claudin-1, and CD81 versus SR-BI in HCV-infected (P=0.0012, P<0.0001, P=0.0004, and P<0.0001, respectively) and normal livers (P<0.0001, P=0.0051, P<0.0001, and P<0.0001, respectively). Positive correlation was observed between serum levels of HCV core antigen and LDL-C (P=0.0147), with their levels negatively correlated to LDLR (P=0.0270 and P=0.0021, respectively). Immunohistochemically, hepatocellular expression of claudin-1 and occludin was increased in HCV-infected livers. Different levels of expression were demonstrated at the mRNA and protein levels for occludin and claudin-1 in HCV-infected and normal livers. Correlation of elements associated with viral entry was comparable in HCV-infected and normal livers.
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U2 - 10.1002/jmv.22042
DO - 10.1002/jmv.22042
M3 - Article
C2 - 21412800
AN - SCOPUS:79952726745
SN - 0146-6615
VL - 83
SP - 921
EP - 927
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 5
ER -
