TY - JOUR
T1 - Expression of the c-myc and c-fos genes as a potential indicator of late radiation damage to the kidney
AU - Otsuka, Makoto
AU - Hatakenaka, Masamitsu
AU - Ishigami, Kousei
AU - Masuda, Kouji
N1 - Funding Information:
We would like to thank Ms. Junko Takano for her excellent technical assistance. This work was partly supported by Grants-in-Aid for Scientific Research (C) 08671039 and 10670858 from the Japanese Ministry of Education, Science, Sports and Culture.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Purpose: To evaluate proliferative response according to c-myc and c-fos gene expression as an early indicator of late radiation damage to the kidney. Methods and Materials: c-myc and c-fos gene expression was measured in the irradiated kidney of C3H/HeSlc mice using reverse transcriptase-polymerase chain reaction (RT-PCR) 24 h after 9, 12, and 15 Gy as well as 2 and 7 days after 15 Gy. In a second experiment, 12 Gy was administered to the right kidney plus the lower half of left kidney. The mice underwent left nephrectomy 24 h after, and the correlation between increased gene expression and elevation of the blood urea nitrogen (BUN) level representing late functional damage was investigated. Results: c-myc and c-fos expression increased in 1 of 10 controls and in 8 of 10 and 7 of 10 mice that received 15 Gy, respectively. Expression increased dose-dependently from 9 to 15 Gy. In mice having an increased BUN level, 7 of 10 and 8 of 10 were positive for c-myc and c-fos, respectively. All 6 mice having an unchanged BUN level were negative for c-fos, while 5 were negative for c-myc. Conclusion: Measurement of c-myc and c-fos gene expression is potentially an early indicator of late radiation damage to the kidney.
AB - Purpose: To evaluate proliferative response according to c-myc and c-fos gene expression as an early indicator of late radiation damage to the kidney. Methods and Materials: c-myc and c-fos gene expression was measured in the irradiated kidney of C3H/HeSlc mice using reverse transcriptase-polymerase chain reaction (RT-PCR) 24 h after 9, 12, and 15 Gy as well as 2 and 7 days after 15 Gy. In a second experiment, 12 Gy was administered to the right kidney plus the lower half of left kidney. The mice underwent left nephrectomy 24 h after, and the correlation between increased gene expression and elevation of the blood urea nitrogen (BUN) level representing late functional damage was investigated. Results: c-myc and c-fos expression increased in 1 of 10 controls and in 8 of 10 and 7 of 10 mice that received 15 Gy, respectively. Expression increased dose-dependently from 9 to 15 Gy. In mice having an increased BUN level, 7 of 10 and 8 of 10 were positive for c-myc and c-fos, respectively. All 6 mice having an unchanged BUN level were negative for c-fos, while 5 were negative for c-myc. Conclusion: Measurement of c-myc and c-fos gene expression is potentially an early indicator of late radiation damage to the kidney.
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U2 - 10.1016/S0360-3016(00)01371-7
DO - 10.1016/S0360-3016(00)01371-7
M3 - Article
C2 - 11163511
AN - SCOPUS:0035162183
SN - 0360-3016
VL - 49
SP - 169
EP - 173
JO - International Journal of Radiation Oncology Biology Physics
JF - International Journal of Radiation Oncology Biology Physics
IS - 1
ER -
