Purpose. Retinal nccn'asculanzatior is a critical event leading to lision loss. Hypoxia is thought to be a common precursor to neovascularization in many retinal disease. Transcription factor N -KB which is activated by hypoxia, is a pleiotropic regulator of many genes including interleukm-8 (IL-8), a potent angiogenic factor. We examined the presence of NF-KB and IL-8, the correlative link between them and their role in ret nal neovascularization. Methods. Using immunohistochemistry. the location and time course of NF-icB and IL-8 expression were studied in a rat mods 1 ol proliferative retmopathy, presumedly caused by relative hypoxia. Results, lctivated NF-icB and IL-8 expression was detected in glial cells in the nerve filer layer and those around the superficial vessels of the retina as u ell as some endothelial cells of neovascular tufts. On the other hand, pericytes and smooth iiuscle cells in the lessel walls expressed NF-icB but noi IL-8. Activated NF-i:B and IL-8 immunoreactivity was most prominently observed near the optic disc, in the nonperfused retinas. Activated NF-KB immunoreactivity was dcterted first, which was followed by IL-8 expression, prior 10 the development cfneovasculanzation. Their expression was marked during ihe development of nccvasculan/ation in comparison to that m the control retinas and then declined as the neovascularization regressed. Conclusions. These data suggest Üat hypoxia could cause NF-icB activation which might induce IL-8 expression, ;.nd that NF-icB and IL-8 play an important role in the development of neovasculai ization.
|Investigative Ophthalmology and Visual Science
|Published - 1997
All Science Journal Classification (ASJC) codes
- Sensory Systems
- Cellular and Molecular Neuroscience