TY - JOUR
T1 - Expression of Id and ITF-2 genes in the mammary gland during pregnancy
AU - Itahana, Yoko
AU - Piens, Marie
AU - Fong, Sylvia
AU - Singh, Jarnail
AU - Sumida, Tomoki
AU - Desprez, Pierre Yves
N1 - Funding Information:
We thank Dr. Ilona Skerjanc (University of Western Ontario) for the ITF-2A and ITF-2B expression vectors, and Dr. Sean McAllister for critical reading of the manuscript. This work was supported by grants from the California Breast Cancer Research Program (7WB-0026) and the National Institutes of Health-National Cancer Institute (RO1 CA82548) to P.Y.D.
PY - 2008/8/8
Y1 - 2008/8/8
N2 - Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands.
AB - Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands.
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U2 - 10.1016/j.bbrc.2008.05.139
DO - 10.1016/j.bbrc.2008.05.139
M3 - Article
C2 - 18519032
AN - SCOPUS:45449092749
SN - 0006-291X
VL - 372
SP - 826
EP - 830
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -