Expression of DCC and netrin-1 in normal human endometrium and its implication in endometrial carcinogenesis

H. D. Kato, H. Kondoh, T. Inoue, K. Asanoma, T. Matsuda, T. Arima, K. Kato, T. Yoshikawa, N. Wake

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Although DCC has been considered as a candidate tumor suppressor, the roles it plays in the uterine endometrium and in the carcinogenic process remains unclear. To define these roles more clearly, we examined the expression of DCC and its ligand, netrin-1, in the normal endometrium and in endometrial cancer. The expression of DCC and netrin-1 in normal endometrial glands and in cancer cell lines was examined by RT-PCR and immunohistochemistry. The effects of exogenous DCC and netrin-1 expression were observed together with the respective expression vector transfection. Endometrial glands in the proliferative and early secretory phase expressed both DCC and netrin-1, but glands in the late-secretory phase tended to silence DCC expression. In addition, all of the endometrial cancer cell lines lost normal DCC expression. Restored DCC expression in the cancer cell lines in the absence of netrin-1 induced apoptosis. However, no changes were observed in the presence of netrin-1. Our observations suggest that DCC/netrin-1 signaling may commit cells to the transition of endometrial gland architecture or function from a proliferating to a secretory phase. In addition, the silencing of DCC expression may contribute to the escape of endometrial cancer cells from a DCC-regulated apoptotic program, thereby promoting malignant phenotypes.

Original languageEnglish
Pages (from-to)281-289
Number of pages9
JournalGynecologic Oncology
Issue number2
Publication statusPublished - Nov 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Obstetrics and Gynaecology


Dive into the research topics of 'Expression of DCC and netrin-1 in normal human endometrium and its implication in endometrial carcinogenesis'. Together they form a unique fingerprint.

Cite this