Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter

Ryoji Fujimaki; Katsuhiko Hayashi; Naoko Watanabe; Taketo Yamada; Yoshiaki Toyama; Ken Ichi Tezuka; Nobumichi Hozumi

doi:10.1007/s00774-004-0595-y

Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter

Ryoji Fujimaki
, Katsuhiko Hayashi
, Naoko Watanabe
, Taketo Yamada
, Yoshiaki Toyama
, Ken Ichi Tezuka
, Nobumichi Hozumi

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.

Original language	English
Pages (from-to)	270-273
Number of pages	4
Journal	Journal of Bone and Mineral Metabolism
Volume	23
Issue number	3
DOIs	https://doi.org/10.1007/s00774-004-0595-y
Publication status	Published - May 2005
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Endocrinology, Diabetes and Metabolism
Orthopedics and Sports Medicine
Endocrinology

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10.1007/s00774-004-0595-y

Cite this

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title = "Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter",

abstract = "Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.",

author = "Ryoji Fujimaki and Katsuhiko Hayashi and Naoko Watanabe and Taketo Yamada and Yoshiaki Toyama and Tezuka, \{Ken Ichi\} and Nobumichi Hozumi",

year = "2005",

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doi = "10.1007/s00774-004-0595-y",

language = "English",

volume = "23",

pages = "270--273",

journal = "Journal of Bone and Mineral Metabolism",

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T1 - Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter

AU - Fujimaki, Ryoji

AU - Hayashi, Katsuhiko

AU - Watanabe, Naoko

AU - Yamada, Taketo

AU - Toyama, Yoshiaki

AU - Tezuka, Ken Ichi

AU - Hozumi, Nobumichi

PY - 2005/5

Y1 - 2005/5

N2 - Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.

AB - Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.

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U2 - 10.1007/s00774-004-0595-y

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M3 - Article

C2 - 15838632

AN - SCOPUS:18044381310

SN - 0914-8779

VL - 23

SP - 270

EP - 273

JO - Journal of Bone and Mineral Metabolism

JF - Journal of Bone and Mineral Metabolism

IS - 3

ER -

Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter

Abstract

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