TY - JOUR
T1 - Expression and function of dopamine in odontoblasts
AU - Fujino, Shoko
AU - Hamano, Sayuri
AU - Tomokiyo, Atsushi
AU - Itoyama, Tomohiro
AU - Hasegawa, Daigaku
AU - Sugii, Hideki
AU - Yoshida, Shinichiro
AU - Washio, Ayako
AU - Nozu, Aoi
AU - Ono, Taiga
AU - Wada, Naohisa
AU - Kitamura, Chiaki
AU - Maeda, Hidefumi
N1 - Funding Information:
The authors acknowledge Drs. Mai Arima, Keita Ipposhi, Kozue Yamashita, Hiroshi Kaneko, Orie Adachi, Masataka Kadowaki, and Risa Sugiura for their support. We also thank Mitchell Arico from Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript. This study was financially supported by KAKENHI grant numbers (JP19K19031, JP17H01598, and JP17H04385) from the Japan Society for the Promotion of Science.
Funding Information:
The authors acknowledge Drs. Mai Arima, Keita Ipposhi, Kozue Yamashita, Hiroshi Kaneko, Orie Adachi, Masataka Kadowaki, and Risa Sugiura for their support. We also thank Mitchell Arico from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript. This study was financially supported by KAKENHI grant numbers (JP19K19031, JP17H01598, and JP17H04385) from the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Dopamine (DA) is produced from tyrosine by tyrosine hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts. However, the role of DA in odontoblasts has not been examined. Therefore, in this investigation, we researched the expression of TH and DA in odontoblasts and the effects of DA on the differentiation of preodontoblasts (KN-3 cells). Immunostaining showed that TH and DA were intensely expressed in odontoblasts and preodontoblasts of rat incisors and molars. KN-3 cells expressed D1-like and D2-like receptors for DA. Furthermore, DA promoted odontoblastic differentiation of KN-3 cells, whereas an antagonist of D1-like receptors and a PKA signaling blocker, inhibited such differentiation. However, antagonists of D2-like receptors promoted differentiation. These results suggested that DA in preodontoblasts and odontoblasts might promote odontoblastic differentiation through D1-like receptors, but not D2-like receptors, and PKA signaling in an autocrine or paracrine manner and plays roles in dentinogenesis.
AB - Dopamine (DA) is produced from tyrosine by tyrosine hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts. However, the role of DA in odontoblasts has not been examined. Therefore, in this investigation, we researched the expression of TH and DA in odontoblasts and the effects of DA on the differentiation of preodontoblasts (KN-3 cells). Immunostaining showed that TH and DA were intensely expressed in odontoblasts and preodontoblasts of rat incisors and molars. KN-3 cells expressed D1-like and D2-like receptors for DA. Furthermore, DA promoted odontoblastic differentiation of KN-3 cells, whereas an antagonist of D1-like receptors and a PKA signaling blocker, inhibited such differentiation. However, antagonists of D2-like receptors promoted differentiation. These results suggested that DA in preodontoblasts and odontoblasts might promote odontoblastic differentiation through D1-like receptors, but not D2-like receptors, and PKA signaling in an autocrine or paracrine manner and plays roles in dentinogenesis.
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U2 - 10.1002/jcp.29314
DO - 10.1002/jcp.29314
M3 - Article
C2 - 31612496
AN - SCOPUS:85074082563
SN - 0021-9541
VL - 235
SP - 4376
EP - 4387
JO - Journal of cellular physiology
JF - Journal of cellular physiology
IS - 5
ER -