Excitatory effects of ATP on rat dorsomedial hypothalamic neurons

N. Matsumoto; M. Sorimachi; N. Akaike

doi:10.1016/j.brainres.2004.03.001

Excitatory effects of ATP on rat dorsomedial hypothalamic neurons

N. Matsumoto, M. Sorimachi, N. Akaike

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Abstract

We investigated P2X purinoceptors in rat dorsomedial hypothalamic (DMH) neurons using nystatin-perforated patch-clamp recordings and fura-2 microfluorometry. Adenosine triphosphate (ATP) concentration-dependently evoked an inward current and increased cytosolic Ca²⁺ ([Ca]_i). The rise in [Ca]_i was dependent on external Ca²⁺ and Na⁺, was blocked by Ca²⁺ channel antagonists and had pharmacological properties consistent with P2X2 receptors. These results suggest that P2X receptor-mediated depolarization activates voltage-gated Ca ²⁺ channels, resulting in an increase in [Ca]_i.

Original language	English
Pages (from-to)	234-237
Number of pages	4
Journal	Brain Research
Volume	1009
Issue number	1-2
DOIs	https://doi.org/10.1016/j.brainres.2004.03.001
Publication status	Published - May 29 2004

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Excitatory effects of ATP on rat dorsomedial hypothalamic neurons",

abstract = "We investigated P2X purinoceptors in rat dorsomedial hypothalamic (DMH) neurons using nystatin-perforated patch-clamp recordings and fura-2 microfluorometry. Adenosine triphosphate (ATP) concentration-dependently evoked an inward current and increased cytosolic Ca2+ ([Ca]i). The rise in [Ca]i was dependent on external Ca2+ and Na+, was blocked by Ca2+ channel antagonists and had pharmacological properties consistent with P2X2 receptors. These results suggest that P2X receptor-mediated depolarization activates voltage-gated Ca 2+ channels, resulting in an increase in [Ca]i.",

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AB - We investigated P2X purinoceptors in rat dorsomedial hypothalamic (DMH) neurons using nystatin-perforated patch-clamp recordings and fura-2 microfluorometry. Adenosine triphosphate (ATP) concentration-dependently evoked an inward current and increased cytosolic Ca2+ ([Ca]i). The rise in [Ca]i was dependent on external Ca2+ and Na+, was blocked by Ca2+ channel antagonists and had pharmacological properties consistent with P2X2 receptors. These results suggest that P2X receptor-mediated depolarization activates voltage-gated Ca 2+ channels, resulting in an increase in [Ca]i.

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Excitatory effects of ATP on rat dorsomedial hypothalamic neurons

Abstract

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