Examination of the usefulness of next-generation sequencing in mixed DNA samples

Fumi Momota; Akiko Tsuji; Atsushi Ishiko; Noriaki Ikeda

doi:10.1016/j.legalmed.2021.101874

Examination of the usefulness of next-generation sequencing in mixed DNA samples

Fumi Momota, Akiko Tsuji, Atsushi Ishiko, Noriaki Ikeda

Social Medicine

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The identification of individuals from mixed DNA samples is an important application of DNA typing. Although the discriminatory power of DNA profiling has improved dramatically, a limiting factor is that individuals cannot be identified via short tandem repeat (STR) analysis. We used next-generation sequencing (NGS) to examine the mixed DNA samples. Our results showed that STR nucleotide sequences and single nucleotide polymorphisms (SNPs) analysis via NGS may enable the identification of each distinct subject from a DNA mixture containing DNA of the victim and suspect.

Original language	English
Article number	101874
Journal	Legal Medicine
Volume	51
DOIs	https://doi.org/10.1016/j.legalmed.2021.101874
Publication status	Published - Jul 2021

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Issues, ethics and legal aspects

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10.1016/j.legalmed.2021.101874

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title = "Examination of the usefulness of next-generation sequencing in mixed DNA samples",

abstract = "The identification of individuals from mixed DNA samples is an important application of DNA typing. Although the discriminatory power of DNA profiling has improved dramatically, a limiting factor is that individuals cannot be identified via short tandem repeat (STR) analysis. We used next-generation sequencing (NGS) to examine the mixed DNA samples. Our results showed that STR nucleotide sequences and single nucleotide polymorphisms (SNPs) analysis via NGS may enable the identification of each distinct subject from a DNA mixture containing DNA of the victim and suspect.",

author = "Fumi Momota and Akiko Tsuji and Atsushi Ishiko and Noriaki Ikeda",

note = "Funding Information: The English used in this manuscript was revised by editage. This study was approved by the Ethics Committee of the Japanese Association of Forensic Science and Technology (The approval number is 29G12, 30GR3). Informed consent was obtained from all individual participating in this study. The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper. Publisher Copyright: {\textcopyright} 2021",

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AU - Momota, Fumi

AU - Tsuji, Akiko

AU - Ishiko, Atsushi

AU - Ikeda, Noriaki

N1 - Funding Information: The English used in this manuscript was revised by editage. This study was approved by the Ethics Committee of the Japanese Association of Forensic Science and Technology (The approval number is 29G12, 30GR3). Informed consent was obtained from all individual participating in this study. The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper. Publisher Copyright: © 2021

PY - 2021/7

Y1 - 2021/7

N2 - The identification of individuals from mixed DNA samples is an important application of DNA typing. Although the discriminatory power of DNA profiling has improved dramatically, a limiting factor is that individuals cannot be identified via short tandem repeat (STR) analysis. We used next-generation sequencing (NGS) to examine the mixed DNA samples. Our results showed that STR nucleotide sequences and single nucleotide polymorphisms (SNPs) analysis via NGS may enable the identification of each distinct subject from a DNA mixture containing DNA of the victim and suspect.

AB - The identification of individuals from mixed DNA samples is an important application of DNA typing. Although the discriminatory power of DNA profiling has improved dramatically, a limiting factor is that individuals cannot be identified via short tandem repeat (STR) analysis. We used next-generation sequencing (NGS) to examine the mixed DNA samples. Our results showed that STR nucleotide sequences and single nucleotide polymorphisms (SNPs) analysis via NGS may enable the identification of each distinct subject from a DNA mixture containing DNA of the victim and suspect.

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Examination of the usefulness of next-generation sequencing in mixed DNA samples

Abstract

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