Evidence for hepatocyte differentiation from embryonic stem cells in vitro

H. Miyashita; A. Suzuki; K. Fukao; H. Nakauchi; H. Taniguchi

doi:10.3727/000000002783985675

Evidence for hepatocyte differentiation from embryonic stem cells in vitro

H. Miyashita, A. Suzuki, K. Fukao, H. Nakauchi, H. Taniguchi

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Abstract

We confirmed hepatocyte differentiation from embryonic stem (ES) cells in vitro. RT-PCR analysis revealed that a broad range of hepatic gene expression was observed in ES cells differentiated through formation of embryoid bodies (EBs) and its attachment culture. Quantitative PCR analysis revealed that hepatic gene expression related to early and late-stage liver development were enhanced through in vitro differentiation of ES cells. The presence of albumin-producing cells in the peripheral region of attached EBs was confirmed by immunocytochemical analysis. Future experiments will reveal the molecules that induce hepatocyte differentiation from ES cells in vitro. This research will provide systems for the investigation of mechanisms in liver development and establish a method of ES cell-based therapy for liver diseases.

Original language	English
Pages (from-to)	429-434
Number of pages	6
Journal	Cell Transplantation
Volume	11
Issue number	5
DOIs	https://doi.org/10.3727/000000002783985675
Publication status	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biomedical Engineering
Cell Biology
Transplantation

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AU - Suzuki, A.

AU - Fukao, K.

AU - Nakauchi, H.

AU - Taniguchi, H.

PY - 2002

Y1 - 2002

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AB - We confirmed hepatocyte differentiation from embryonic stem (ES) cells in vitro. RT-PCR analysis revealed that a broad range of hepatic gene expression was observed in ES cells differentiated through formation of embryoid bodies (EBs) and its attachment culture. Quantitative PCR analysis revealed that hepatic gene expression related to early and late-stage liver development were enhanced through in vitro differentiation of ES cells. The presence of albumin-producing cells in the peripheral region of attached EBs was confirmed by immunocytochemical analysis. Future experiments will reveal the molecules that induce hepatocyte differentiation from ES cells in vitro. This research will provide systems for the investigation of mechanisms in liver development and establish a method of ES cell-based therapy for liver diseases.

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Evidence for hepatocyte differentiation from embryonic stem cells in vitro

Abstract

All Science Journal Classification (ASJC) codes

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