The potential of different housekeeping genes for their use as internal controls of gene expression under changing environmental conditions was investigated in hyacinth (Hyacinthus orientalis L.) bulbs during storage at low (5°C) and room temperatures (25°C) and culture period at 25°C. Proper annealing temperatures for the expression analysis of genes for actin were between 50 and 63°C, and proper PCR cycles for actin and 18S rRNA were 30 and 25, respectively. The expression of actin increased during cold storage period and unstable during culture, whereas that of 18S rRNA was kept unchanged throughout the storage and culture periods. These results indicated that 18S rRNA was more reliable than actin as an internal control for semi-quantitative RT-PCR of total RNA in hyacinth. This is the first report on the establishment of the internal standard for the studies on expression levels of genes in bulbous plants.
|Number of pages
|Journal of the Faculty of Agriculture, Kyushu University
|Published - Feb 2005
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science