TY - GEN
T1 - Establishment of portable immunoassay system for early diagnosis
AU - Kasama, Toshihiro
AU - Hasegawa, Yutaka
AU - Matsumoto, Hiroyuki
AU - Kondo, Haruyasu
AU - Ozawa, Tsutomu
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2013
Y1 - 2013
N2 - We have developed immunopillar devices for rapid and easy-to-use immunoassay with pM-fM detection sensitivity, but long total assay time (sample-in-answer-out) and the compatibility with a portable detection system have still remained as major problems toward the point-of-care testing (POCT). We report here next generation immunopillar devices and portable detection system suitable for them. Thin structure of those devices allowed us to wash nonspecifically bound antigens and fluorescent-labeled secondary antibodies in a minute, resulting in the dramatic reduction in the total assay time. In addition, by using the portable detection system, we gained the concentrations of C-reactive protein in human sera while preserving pM detection sensitivity. The total assay time was 20 minutes per sample. Our immunoassay system possesses the potential for use in POCT.
AB - We have developed immunopillar devices for rapid and easy-to-use immunoassay with pM-fM detection sensitivity, but long total assay time (sample-in-answer-out) and the compatibility with a portable detection system have still remained as major problems toward the point-of-care testing (POCT). We report here next generation immunopillar devices and portable detection system suitable for them. Thin structure of those devices allowed us to wash nonspecifically bound antigens and fluorescent-labeled secondary antibodies in a minute, resulting in the dramatic reduction in the total assay time. In addition, by using the portable detection system, we gained the concentrations of C-reactive protein in human sera while preserving pM detection sensitivity. The total assay time was 20 minutes per sample. Our immunoassay system possesses the potential for use in POCT.
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U2 - 10.1109/MHS.2013.6710451
DO - 10.1109/MHS.2013.6710451
M3 - Conference contribution
AN - SCOPUS:84893779922
SN - 9781479915293
T3 - 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
BT - 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
PB - IEEE Computer Society
T2 - 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
Y2 - 10 November 2013 through 13 November 2013
ER -