TY - JOUR
T1 - Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse
T2 - In vivo differentiation into lymphocytes and potential for germ line transmission
AU - Nagafuchi, Seiho
AU - Katsuta, Hitoshi
AU - Kogawa, Kazuhiko
AU - Akashi, Tomoyuki
AU - Kondo, Shiori
AU - Sakai, Yumiko
AU - Tsukiyama, Tadasuke
AU - Kitamura, Daisuke
AU - Niho, Yoshiyuki
AU - Watanabe, Takeshi
N1 - Funding Information:
The authors thank Yukari Yamada, Laboratory of Embryonic and Genetic Engineering, Medical Institute of Bioregulation, Kyushu University, for her excellent technical assistance. We also express our sincere thanks to Dr. Peter D. Burrows for his critical reading of the manuscript. This work is supported in part by a grant from Kyushu University Interdisciplinary Programs in Education and Projects in Research Development.
PY - 1999/7/16
Y1 - 1999/7/16
N2 - A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(β)(g7) haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(β)(g7) haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. Copyright (C) 1999 Federation of European Biochemical Societies.
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U2 - 10.1016/S0014-5793(99)00801-7
DO - 10.1016/S0014-5793(99)00801-7
M3 - Article
C2 - 10428481
AN - SCOPUS:0033035929
SN - 0014-5793
VL - 455
SP - 101
EP - 104
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -
