Establishment of a novel method of screening anti-Allergic lactic acid bacteria

Keishi Kadooka; Asuka Imahayashi; Ayaka Koiso; Makiko Yamashita; Kiichiro Teruya; Takashi Matsumoto; Takanori Hasegawa; Fumiki Morimatsu; Yoshinori Katakura

doi:10.1271/bbb.110069

Establishment of a novel method of screening anti-Allergic lactic acid bacteria

Keishi Kadooka, Asuka Imahayashi, Ayaka Koiso, Makiko Yamashita, Kiichiro Teruya, Takashi Matsumoto, Takanori Hasegawa, Fumiki Morimatsu, Yoshinori Katakura

Systems Biology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

In this study, we attempted to establish a novel method of screening anti-allergic lactic acid bacteria (LAB). We cloned the human histidine decarboxylase (HDC) promoter into the promoter-less pPhi-Yellow-RPL-dest1 vector and established KU812F cells transduced with this vector (pHDCp-Phi-Yellow/ KU812F). After adding LAB to these cells, the change in fluorescence intensity was monitored by flow cytometry. After screening, we identified several LAB strains that downregulated HDC promoter activity. Functional analysis of these LAB strains indicated that two LAB strains inhibited histamine release from KU812F cells, indicating that this assay system can be used to screen for anti-allergic LAB in a high-throughput manner.

Original language	English
Pages (from-to)	1016-1018
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	75
Issue number	5
DOIs	https://doi.org/10.1271/bbb.110069
Publication status	Published - 2011

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1271/bbb.110069

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title = "Establishment of a novel method of screening anti-Allergic lactic acid bacteria",

abstract = "In this study, we attempted to establish a novel method of screening anti-allergic lactic acid bacteria (LAB). We cloned the human histidine decarboxylase (HDC) promoter into the promoter-less pPhi-Yellow-RPL-dest1 vector and established KU812F cells transduced with this vector (pHDCp-Phi-Yellow/ KU812F). After adding LAB to these cells, the change in fluorescence intensity was monitored by flow cytometry. After screening, we identified several LAB strains that downregulated HDC promoter activity. Functional analysis of these LAB strains indicated that two LAB strains inhibited histamine release from KU812F cells, indicating that this assay system can be used to screen for anti-allergic LAB in a high-throughput manner.",

author = "Keishi Kadooka and Asuka Imahayashi and Ayaka Koiso and Makiko Yamashita and Kiichiro Teruya and Takashi Matsumoto and Takanori Hasegawa and Fumiki Morimatsu and Yoshinori Katakura",

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AU - Kadooka, Keishi

AU - Imahayashi, Asuka

AU - Koiso, Ayaka

AU - Yamashita, Makiko

AU - Teruya, Kiichiro

AU - Matsumoto, Takashi

AU - Hasegawa, Takanori

AU - Morimatsu, Fumiki

AU - Katakura, Yoshinori

PY - 2011

Y1 - 2011

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AB - In this study, we attempted to establish a novel method of screening anti-allergic lactic acid bacteria (LAB). We cloned the human histidine decarboxylase (HDC) promoter into the promoter-less pPhi-Yellow-RPL-dest1 vector and established KU812F cells transduced with this vector (pHDCp-Phi-Yellow/ KU812F). After adding LAB to these cells, the change in fluorescence intensity was monitored by flow cytometry. After screening, we identified several LAB strains that downregulated HDC promoter activity. Functional analysis of these LAB strains indicated that two LAB strains inhibited histamine release from KU812F cells, indicating that this assay system can be used to screen for anti-allergic LAB in a high-throughput manner.

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Establishment of a novel method of screening anti-Allergic lactic acid bacteria

Abstract

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