TY - JOUR
T1 - Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma
AU - Sekino, Yuki
AU - Imaizumi, Akira
AU - Komune, Noritaka
AU - Ono, Mayumi
AU - Sato, Kuniaki
AU - Masuda, Shogo
AU - Fujimura, Akiko
AU - Koike, Kensuke
AU - Hongo, Takahiro
AU - Uchi, Ryutaro
AU - Onishi, Hideya
AU - Nakagawa, Takashi
N1 - Funding Information:
The authors would like to thank Japan Medical Communication and Uni-edit for editing our manuscript. Part of this work was supported by JSPS KAKENHI Grant Number JP 18H02951 and 18K16895.
Funding Information:
The authors would like to thank Japan Medical Communication and Uni‐edit for editing our manuscript. Part of this work was supported by JSPS KAKENHI Grant Number JP 18H02951 and 18K16895.
Publisher Copyright:
© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
PY - 2021/8
Y1 - 2021/8
N2 - There are no human cancer cell lines of external auditory canal origin available for research use. This report describes the establishment of a culture condition for external auditory canal squamous cell carcinoma, derived from human tumor tissue. Successive squamous cell carcinoma colonies were dissociated by trypsin, subcultured, and maintained on a feeder layer (MMC-TIG-1-20), yielding a clonally proliferating cell culture. Two morphological types of colony were observed: (a) densely packed colonies and (b) colonies with indistinct boundaries characterized by cell–cell complexes with fibroblast feeder cells. The SCC-like characteristics of these cells were evidenced by positivity for p53, SCCA1/2, cytokeratin, and vimentin, and cancer stem cell properties were indicated by positivity for CD44, CD133, Oct3/4, and alkaline phosphatase (ALP). One of the unique properties of cell cultures is their tendency to form steric colonies in vitro on feeder layer cells. In addition, in the presence of fresh macrophages, the cells very slowly transform to break away from colonies as free cells, a process that resembles the epidermal–mesenchymal transition, whereby cell–cell interactions are weakened and migration activity is enhanced. These factors are purported to play a key role in cancer cell metastasis.
AB - There are no human cancer cell lines of external auditory canal origin available for research use. This report describes the establishment of a culture condition for external auditory canal squamous cell carcinoma, derived from human tumor tissue. Successive squamous cell carcinoma colonies were dissociated by trypsin, subcultured, and maintained on a feeder layer (MMC-TIG-1-20), yielding a clonally proliferating cell culture. Two morphological types of colony were observed: (a) densely packed colonies and (b) colonies with indistinct boundaries characterized by cell–cell complexes with fibroblast feeder cells. The SCC-like characteristics of these cells were evidenced by positivity for p53, SCCA1/2, cytokeratin, and vimentin, and cancer stem cell properties were indicated by positivity for CD44, CD133, Oct3/4, and alkaline phosphatase (ALP). One of the unique properties of cell cultures is their tendency to form steric colonies in vitro on feeder layer cells. In addition, in the presence of fresh macrophages, the cells very slowly transform to break away from colonies as free cells, a process that resembles the epidermal–mesenchymal transition, whereby cell–cell interactions are weakened and migration activity is enhanced. These factors are purported to play a key role in cancer cell metastasis.
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U2 - 10.1002/2211-5463.13225
DO - 10.1002/2211-5463.13225
M3 - Article
C2 - 34115931
AN - SCOPUS:85108795756
SN - 2211-5463
VL - 11
SP - 2211
EP - 2224
JO - FEBS Open Bio
JF - FEBS Open Bio
IS - 8
ER -
