TY - JOUR
T1 - Establishment and characterization of a novel orthotopic mouse model for human uterine sarcoma with different metastatic potentials
AU - Kawabe, Shinya
AU - Mizutani, Tetsuya
AU - Ishikane, Shin
AU - Martinez, Miguel Ernesto
AU - Kiyono, Yasushi
AU - Miura, Koichi
AU - Hosoda, Hiroshi
AU - Imamichi, Yoshitaka
AU - Kangawa, Kenji
AU - Miyamoto, Kaoru
AU - Yoshida, Yoshio
N1 - Publisher Copyright:
© 2015 Elsevier Ireland Ltd.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Uterine sarcomas are rare and aggressive gynecologic tumors with a poor prognosis because of recurrence and metastasis. However, the mechanisms of uterine sarcoma metastasis are largely unknown. To investigate this mechanism, we developed a novel uterine sarcoma tissue-derived orthotopic and metastatic model in KSN nude mice using a green fluorescent protein stably expressed uterine sarcoma cell line, MES-SA. Histological analysis showed that all orthotopic primary tumors were undifferentiated sarcoma. Primary tumors were characterized by high 18F-fluorodeoxyglucose uptake with a positive correlation to the number of pulmonary metastases. In addition, we generated uterine sarcoma cell sublines with high or low metastatic potentials by serial in vivo selection. Microarray analysis between orthotopic tumors with high and low metastatic potentials revealed differential expression of genes related to cell proliferation and migration (TNNT1, COL1A2, and ZIC1). Our model would be useful to compensate for the limited clinical cases of uterine sarcoma and to investigate the molecular mechanisms of metastatic uterine sarcoma.
AB - Uterine sarcomas are rare and aggressive gynecologic tumors with a poor prognosis because of recurrence and metastasis. However, the mechanisms of uterine sarcoma metastasis are largely unknown. To investigate this mechanism, we developed a novel uterine sarcoma tissue-derived orthotopic and metastatic model in KSN nude mice using a green fluorescent protein stably expressed uterine sarcoma cell line, MES-SA. Histological analysis showed that all orthotopic primary tumors were undifferentiated sarcoma. Primary tumors were characterized by high 18F-fluorodeoxyglucose uptake with a positive correlation to the number of pulmonary metastases. In addition, we generated uterine sarcoma cell sublines with high or low metastatic potentials by serial in vivo selection. Microarray analysis between orthotopic tumors with high and low metastatic potentials revealed differential expression of genes related to cell proliferation and migration (TNNT1, COL1A2, and ZIC1). Our model would be useful to compensate for the limited clinical cases of uterine sarcoma and to investigate the molecular mechanisms of metastatic uterine sarcoma.
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U2 - 10.1016/j.canlet.2015.06.018
DO - 10.1016/j.canlet.2015.06.018
M3 - Article
C2 - 26164209
AN - SCOPUS:84937895196
SN - 0304-3835
VL - 366
SP - 182
EP - 190
JO - Cancer Letters
JF - Cancer Letters
IS - 2
ER -
