TY - JOUR
T1 - Equilibrium shift by target DNA substrates for determination of DNA binding ligands
AU - Tsujita, Saori
AU - Tanada, Mikimasa
AU - Kataoka, Tomonobu
AU - Sasaki, Shigeki
N1 - Funding Information:
This work has been supported by a Grant-in Aid for Scientific Research (A) from Japan Society for the Promotion of Science (JSPS), and CREST from Japan Science and Technology Agency (JST).
PY - 2007/1/1
Y1 - 2007/1/1
N2 - An equilibrium containing the thiol derivative of Hoechst33258 (Ht-SH), glutathione (G-SH), and the corresponding homo and hetero disulfides was shifted by the addition of the duplex DNA. It was shown from the analysis of the components that the hetero disulfide Ht-SS-G increased by binding with the DNA (CA14) with an A3T3 binding motif for the structure of Hoechst33258, and that the different equilibrium shift was observed in the presence of CT14 with no A3T3 binding motif.
AB - An equilibrium containing the thiol derivative of Hoechst33258 (Ht-SH), glutathione (G-SH), and the corresponding homo and hetero disulfides was shifted by the addition of the duplex DNA. It was shown from the analysis of the components that the hetero disulfide Ht-SS-G increased by binding with the DNA (CA14) with an A3T3 binding motif for the structure of Hoechst33258, and that the different equilibrium shift was observed in the presence of CT14 with no A3T3 binding motif.
UR - http://www.scopus.com/inward/record.url?scp=33845616626&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845616626&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2006.09.089
DO - 10.1016/j.bmcl.2006.09.089
M3 - Article
C2 - 17046249
AN - SCOPUS:33845616626
SN - 0960-894X
VL - 17
SP - 68
EP - 72
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 1
ER -