TY - GEN
T1 - Epigenetic analysis of a single DNA molecule by microfluidic device with quantum dot
AU - Sano, Tatsuki
AU - Okamoto, Yukihiro
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2011
Y1 - 2011
N2 - We report a rapid and simple epigenetic analysis based on microfluidic single DNA molecular methylation detection. We developed a microfluidic device to stretch a single DNA molecule and subsequent detection of a methylated DNA base by quantum dot (QD)-immobilized methyl binding domain (MBD) protein. A QD-MBD complex specifically bound to a methylated base of stretched DNA and was detected by a total internal reflection fluorescence microscope (TIRFM). Our method enables us to detect a number of methylation sites and identify the positions methylated simultaneously in the simple and rapid manner compared with the conventional methods.
AB - We report a rapid and simple epigenetic analysis based on microfluidic single DNA molecular methylation detection. We developed a microfluidic device to stretch a single DNA molecule and subsequent detection of a methylated DNA base by quantum dot (QD)-immobilized methyl binding domain (MBD) protein. A QD-MBD complex specifically bound to a methylated base of stretched DNA and was detected by a total internal reflection fluorescence microscope (TIRFM). Our method enables us to detect a number of methylation sites and identify the positions methylated simultaneously in the simple and rapid manner compared with the conventional methods.
UR - http://www.scopus.com/inward/record.url?scp=84883792740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883792740&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883792740
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 299
EP - 301
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -