Enzyme-mediated protein refolding

Jun Okada; Tatsuo Maruyama; Konomi Motomura; Kimiko Kuroki; Katsumi Maenaka; Masafumi Sakono; Masahiro Goto

doi:10.1039/b916225a

Enzyme-mediated protein refolding

Jun Okada, Tatsuo Maruyama, Konomi Motomura, Kimiko Kuroki, Katsumi Maenaka, Masafumi Sakono, Masahiro Goto

Pharmaceutical Health Care and Sciences Faculty

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

Original language	English
Pages (from-to)	7197-7199
Number of pages	3
Journal	Chemical Communications
Issue number	46
DOIs	https://doi.org/10.1039/b916225a
Publication status	Published - 2009

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/b916225a

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title = "Enzyme-mediated protein refolding",

abstract = "We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.",

author = "Jun Okada and Tatsuo Maruyama and Konomi Motomura and Kimiko Kuroki and Katsumi Maenaka and Masafumi Sakono and Masahiro Goto",

year = "2009",

doi = "10.1039/b916225a",

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journal = "Chemical Communications",

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T1 - Enzyme-mediated protein refolding

AU - Okada, Jun

AU - Maruyama, Tatsuo

AU - Motomura, Konomi

AU - Kuroki, Kimiko

AU - Maenaka, Katsumi

AU - Sakono, Masafumi

AU - Goto, Masahiro

PY - 2009

Y1 - 2009

N2 - We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

AB - We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

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DO - 10.1039/b916225a

M3 - Article

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AN - SCOPUS:70450158750

SN - 1359-7345

SP - 7197

EP - 7199

JO - Chemical Communications

JF - Chemical Communications

IS - 46

ER -

Enzyme-mediated protein refolding

Abstract

All Science Journal Classification (ASJC) codes

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