Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies

Benyakan Pongkitwitoon; Seiichi Sakamoto; Hiroyuki Tanaka; Ryota Tsuchihashi; Junei Kinjo; Satoshi Morimoto; Waraporn Putalun

doi:10.1055/s-0029-1240725

Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies

Benyakan Pongkitwitoon, Seiichi Sakamoto, Hiroyuki Tanaka, Ryota Tsuchihashi, Junei Kinjo, Satoshi Morimoto, Waraporn Putalun

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.056.25g/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.

Original language	English
Pages (from-to)	831-836
Number of pages	6
Journal	Planta medica
Volume	76
Issue number	8
DOIs	https://doi.org/10.1055/s-0029-1240725
Publication status	Published - Jun 4 2010

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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title = "Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies",

abstract = "Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.056.25g/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.",

author = "Benyakan Pongkitwitoon and Seiichi Sakamoto and Hiroyuki Tanaka and Ryota Tsuchihashi and Junei Kinjo and Satoshi Morimoto and Waraporn Putalun",

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AU - Pongkitwitoon, Benyakan

AU - Sakamoto, Seiichi

AU - Tanaka, Hiroyuki

AU - Tsuchihashi, Ryota

AU - Kinjo, Junei

AU - Morimoto, Satoshi

AU - Putalun, Waraporn

PY - 2010/6/4

Y1 - 2010/6/4

N2 - Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.056.25g/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.

AB - Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.056.25g/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.

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IS - 8

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Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies

Abstract

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