TY - JOUR
T1 - Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites
AU - Sakamoto, Seiichi
AU - Putalun, Waraporn
AU - Vimolmangkang, Sornkanok
AU - Phoolcharoen, Waranyoo
AU - Shoyama, Yukihiro
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
N1 - Funding Information:
Acknowledgement This work was funded by a Grant-in-Aid for Young Scientists (B) [17K15466] of the Japan Society for the Promotion of Science (JSPS). This work was also supported by the Chulalongkorn visiting professor grants.
Publisher Copyright:
© 2017, The Author(s).
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen–antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies by drug monitoring, and the quality control of commercially available products. Berson and Yalow were the first to develop an immunoassay, known as radioimmunoassay (RIA), for detecting endogenous plasma insulin [1], a development for which Yalow was awarded the Nobel Prize in Physiology or Medicine in 1977. Even today, after half a century, immunoassays are widely utilized with some modifications from the originally proposed system, e.g., radioisotopes have been replaced with enzymes because of safety concerns regarding the use of radioactivity, which is referred to as enzyme immunoassay/enzyme-linked immunosorbent assay (ELISA). In addition, progress has been made in ELISA with the recent advances in recombinant DNA technology, leading to increase in the range of antibodies, probes, and even systems. This review article describes ELISA and its applications for the detection of plant secondary metabolites.
AB - Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen–antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies by drug monitoring, and the quality control of commercially available products. Berson and Yalow were the first to develop an immunoassay, known as radioimmunoassay (RIA), for detecting endogenous plasma insulin [1], a development for which Yalow was awarded the Nobel Prize in Physiology or Medicine in 1977. Even today, after half a century, immunoassays are widely utilized with some modifications from the originally proposed system, e.g., radioisotopes have been replaced with enzymes because of safety concerns regarding the use of radioactivity, which is referred to as enzyme immunoassay/enzyme-linked immunosorbent assay (ELISA). In addition, progress has been made in ELISA with the recent advances in recombinant DNA technology, leading to increase in the range of antibodies, probes, and even systems. This review article describes ELISA and its applications for the detection of plant secondary metabolites.
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U2 - 10.1007/s11418-017-1144-z
DO - 10.1007/s11418-017-1144-z
M3 - Review article
C2 - 29164507
AN - SCOPUS:85034642661
SN - 1340-3443
VL - 72
SP - 32
EP - 42
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 1
ER -