Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Takao Yasui; Koki Motoyama; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

doi:10.1039/c3ra22999h

Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Takao Yasui
, Koki Motoyama
, Noritada Kaji
, Manabu Tokeshi
, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

Original language	English
Pages (from-to)	3237-3240
Number of pages	4
Journal	RSC Advances
Volume	3
Issue number	10
DOIs	https://doi.org/10.1039/c3ra22999h
Publication status	Published - Mar 14 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering

Access to Document

10.1039/c3ra22999h

Cite this

@article{1b28cbc15f5a4a898e352768612fdf55,

title = "Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules",

abstract = "The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.",

author = "Takao Yasui and Koki Motoyama and Noritada Kaji and Manabu Tokeshi and Yoshinobu Baba",

year = "2013",

month = mar,

day = "14",

doi = "10.1039/c3ra22999h",

language = "English",

volume = "3",

pages = "3237--3240",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "Royal Society of Chemistry",

number = "10",

}

TY - JOUR

T1 - Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

AU - Yasui, Takao

AU - Motoyama, Koki

AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

PY - 2013/3/14

Y1 - 2013/3/14

N2 - The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

AB - The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

UR - https://www.scopus.com/pages/publications/84873688461

UR - https://www.scopus.com/pages/publications/84873688461#tab=citedBy

U2 - 10.1039/c3ra22999h

DO - 10.1039/c3ra22999h

M3 - Article

AN - SCOPUS:84873688461

SN - 2046-2069

VL - 3

SP - 3237

EP - 3240

JO - RSC Advances

JF - RSC Advances

IS - 10

ER -

Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this