Enzymatic synthesis of Z-aspartame in liquefied amino acid substrates

Shinya Furukawa, Kazuhiro Hasegawa, Ichiro Fuke, Koji Kittaka, Terumitsu Nakakoba, Masahiro Goto, Noriho Kamiya

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


In this paper, we designed and validated a new media for biotransformation aided by ionic liquefied amino acid substrates. Thermolysin-catalyzed synthesis of Z-aspartame (N-carbobenzoxy-l-aspartame) in which ionic liquids play dual roles as both substrate and reaction medium was conducted. If the protease can retain catalytic activity in this new liquefied amino acid substrate/media, the effective substrate concentration can be maximized, leading to theoretically high yields. In fact, we observed a 34-fold enhancement in the catalytic activity compared with that obtained in a previous report. The yield of Z-aspartame reached at approximately 660mM at 24h with 50mgmL-1 of the enzyme, thus submolar range productivity was achieved. The results suggest the successful construction of a high productivity reaction system for peptide synthesis.

Original languageEnglish
Pages (from-to)84-87
Number of pages4
JournalBiochemical Engineering Journal
Publication statusPublished - Jan 15 2013

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biotechnology
  • Environmental Engineering
  • Biomedical Engineering


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