Enzymatic interesterification of triglyceride with surfactant‐coated lipase in organic media

Masahiro Goto, Muneharu Goto, Noriho Kamiya, Fumiyuki Nakashio

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)


Several surfactant‐coated enzymes have been prepared by coating lipases of various origins with a nonionic surfactant, glutamic acid dioleylester ribitol (2C18Δ9GE). Enzymatic interesterification of tripalmitin with oleic acid using the surfactant‐coated lipase was carried out in organic media. The surfactant‐coated lipases could effectively catalyze the interesterification of glycerides better than did the powder lipases. A suitable organic solvent was an aliphatic hydrocarbon such as isooctane. The enzymatic activity for the interesterification strongly depended on the origin of the lipase. The surfactant‐coated lipase prepared by Mucor javanicus showed the highest enzymatic activity for the interesterification of glycerides, although its powder lipase did not show enzymatic activity. Selective interesterification of glycerides could be performed by adjusting the concentration ratio of oleic acid to tripalmitin in isooctane. Di‐substituted glyceride could be selectively produced when the concentration ratio of carboxylic acid to glycerides was 7. © 1995 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalBiotechnology and Bioengineering
Issue number1
Publication statusPublished - Jan 5 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Enzymatic interesterification of triglyceride with surfactant‐coated lipase in organic media'. Together they form a unique fingerprint.

Cite this