Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media

Noriho Kamiya, Yuichi Matsushita, Misa Hanaki, Kazunori Nakashima, Mamiko Narita, Masahiro Goto, Haruo Takahashi

Research output: Contribution to journalArticlepeer-review

202 Citations (Scopus)


The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40°C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.

Original languageEnglish
Pages (from-to)1037-1040
Number of pages4
JournalBiotechnology letters
Issue number6
Publication statusPublished - Jun 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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