TY - JOUR
T1 - Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media
AU - Kamiya, Noriho
AU - Matsushita, Yuichi
AU - Hanaki, Misa
AU - Nakashima, Kazunori
AU - Narita, Mamiko
AU - Goto, Masahiro
AU - Takahashi, Haruo
PY - 2008/6
Y1 - 2008/6
N2 - The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40°C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.
AB - The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40°C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.
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U2 - 10.1007/s10529-008-9638-0
DO - 10.1007/s10529-008-9638-0
M3 - Article
C2 - 18250967
AN - SCOPUS:42549101431
SN - 0141-5492
VL - 30
SP - 1037
EP - 1040
JO - Biotechnology letters
JF - Biotechnology letters
IS - 6
ER -