TY - JOUR
T1 - Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp
AU - Hokazono, Eisaku
AU - Tamezane, Hideto
AU - Hotta, Taeko
AU - Kayamori, Yuzo
AU - Osawa, Susumu
N1 - Funding Information:
This study was partially supported by Asahi Kasei Pharma, Tokyo, Japan . The authors are indebted to their staff for technical advice and reagents. We are also grateful to the Department of Clinical Laboratory of the Kyushu University Hospital, Fukuoka, Japan, for their donation of blood samples.
PY - 2011/7/15
Y1 - 2011/7/15
N2 - Background: In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. However, it could not measure PE with other phospholipids due to a lack of choline in them. Methods: Using an amine oxidase (EC 1.4.3.6), from Arthrobacter species, a simple and rapid enzymatic assay for measurements of PE in serum was established. That assay used the Hitachi 7170 analyzer to evaluate the analytical performance. Results: The average within-run CVs were 0.38-1.27% (n=20) at 69-160μmol/l. The correlation between values obtained with the present method (y) and the high-performance liquid chromatography (HPLC) method (x) was: y=0.944x+9.441 (r=0.977, Sy|x=5.82, n=34). In addition, the reference interval of healthy subjects was 115±45μmol/l. Conclusions: This new enzymatic method shows a high specificity for serum PE and can be easily applied to an automated analyzer. The present method is available as a novel marker of changes in the clinical condition of serum phospholipids.
AB - Background: In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. However, it could not measure PE with other phospholipids due to a lack of choline in them. Methods: Using an amine oxidase (EC 1.4.3.6), from Arthrobacter species, a simple and rapid enzymatic assay for measurements of PE in serum was established. That assay used the Hitachi 7170 analyzer to evaluate the analytical performance. Results: The average within-run CVs were 0.38-1.27% (n=20) at 69-160μmol/l. The correlation between values obtained with the present method (y) and the high-performance liquid chromatography (HPLC) method (x) was: y=0.944x+9.441 (r=0.977, Sy|x=5.82, n=34). In addition, the reference interval of healthy subjects was 115±45μmol/l. Conclusions: This new enzymatic method shows a high specificity for serum PE and can be easily applied to an automated analyzer. The present method is available as a novel marker of changes in the clinical condition of serum phospholipids.
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U2 - 10.1016/j.cca.2011.04.023
DO - 10.1016/j.cca.2011.04.023
M3 - Article
C2 - 21549106
AN - SCOPUS:79956303929
SN - 0009-8981
VL - 412
SP - 1436
EP - 1440
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 15-16
ER -
