Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays

Masateru Ito, Fumio Nakamura, Akira Baba, Tamada Kaoru, Hirobumi Ushijima, King Hang Aaron Lau, Abhijit Manna, Wolfgang Knoll

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


We have developed a new methodology to fabricate high-resolution DNA microarrays (2500 dots/cm2) in combination with microcontact printing (μCP) and surface plasmon resonance (SPR) imaging. A novel COOHterminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically bound to a gold substrate for surface coupling reactions with NH 2-terminated DNA. The hybridization between target DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfully demonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. The gold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles (∼10%) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancement effect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectric constants and Fresnel's equation.

Original languageEnglish
Pages (from-to)11653-11662
Number of pages10
JournalJournal of Physical Chemistry C
Issue number31
Publication statusPublished - Aug 9 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • General Energy
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films


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