Enhancement of interferon-β production with sphingomyelin from fermented milk

Kazuhiro Osada, Kazuhiko Nagira, Kiichiro Teruya, Hirofumi Tachibana, Sanetaka Shirahata, Hiroki Murakami

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


A fermented milk, Kefir, contains an active substance which enhances IFN-β secretion of a human osteosarcoma line MG-63 treated with a chemical inducer, poly I: poly C. The active substance in the fermented milk was identified to be sphingomyelin (SpM) by a combined use of a fast atom bombardment mass spectrometry (FAB-MS) and a fast atom bombardment tandem mass spectrometry (FAB-MS/MS). SpM from fermented milk (F-SpM) was a mixture of four molecular species of SpMs having C21-, C22-, C23- and C24-fatty acids. F-SpM enhanced the IFN secretion 14 times, SpMs from other sources also enhanced moderately (2-3 times). Sphingosine and lysosphingomyelin also enhanced the activity but ceramide and cerebroside did not.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
Issue number2
Publication statusPublished - Jun 1 1993

All Science Journal Classification (ASJC) codes

  • Pharmacology


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