Enhancement of interferon-β production with sphingomyelin from fermented milk

Kazuhiro Osada; Kazuhiko Nagira; Kiichiro Teruya; Hirofumi Tachibana; Sanetaka Shirahata; Hiroki Murakami

doi:10.1007/BF01877735

Enhancement of interferon-β production with sphingomyelin from fermented milk

Kazuhiro Osada, Kazuhiko Nagira, Kiichiro Teruya, Hirofumi Tachibana, Sanetaka Shirahata, Hiroki Murakami

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

A fermented milk, Kefir, contains an active substance which enhances IFN-β secretion of a human osteosarcoma line MG-63 treated with a chemical inducer, poly I: poly C. The active substance in the fermented milk was identified to be sphingomyelin (SpM) by a combined use of a fast atom bombardment mass spectrometry (FAB-MS) and a fast atom bombardment tandem mass spectrometry (FAB-MS/MS). SpM from fermented milk (F-SpM) was a mixture of four molecular species of SpMs having C₂₁-, C₂₂-, C₂₃- and C₂₄-fatty acids. F-SpM enhanced the IFN secretion 14 times, SpMs from other sources also enhanced moderately (2-3 times). Sphingosine and lysosphingomyelin also enhanced the activity but ceramide and cerebroside did not.

Original language	English
Pages (from-to)	115-123
Number of pages	9
Journal	Biotherapy
Volume	7
Issue number	2
DOIs	https://doi.org/10.1007/BF01877735
Publication status	Published - Jun 1 1993

All Science Journal Classification (ASJC) codes

Pharmacology

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10.1007/BF01877735

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abstract = "A fermented milk, Kefir, contains an active substance which enhances IFN-β secretion of a human osteosarcoma line MG-63 treated with a chemical inducer, poly I: poly C. The active substance in the fermented milk was identified to be sphingomyelin (SpM) by a combined use of a fast atom bombardment mass spectrometry (FAB-MS) and a fast atom bombardment tandem mass spectrometry (FAB-MS/MS). SpM from fermented milk (F-SpM) was a mixture of four molecular species of SpMs having C21-, C22-, C23- and C24-fatty acids. F-SpM enhanced the IFN secretion 14 times, SpMs from other sources also enhanced moderately (2-3 times). Sphingosine and lysosphingomyelin also enhanced the activity but ceramide and cerebroside did not.",

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AU - Osada, Kazuhiro

AU - Nagira, Kazuhiko

AU - Teruya, Kiichiro

AU - Tachibana, Hirofumi

AU - Shirahata, Sanetaka

AU - Murakami, Hiroki

PY - 1993/6/1

Y1 - 1993/6/1

N2 - A fermented milk, Kefir, contains an active substance which enhances IFN-β secretion of a human osteosarcoma line MG-63 treated with a chemical inducer, poly I: poly C. The active substance in the fermented milk was identified to be sphingomyelin (SpM) by a combined use of a fast atom bombardment mass spectrometry (FAB-MS) and a fast atom bombardment tandem mass spectrometry (FAB-MS/MS). SpM from fermented milk (F-SpM) was a mixture of four molecular species of SpMs having C21-, C22-, C23- and C24-fatty acids. F-SpM enhanced the IFN secretion 14 times, SpMs from other sources also enhanced moderately (2-3 times). Sphingosine and lysosphingomyelin also enhanced the activity but ceramide and cerebroside did not.

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Enhancement of interferon-β production with sphingomyelin from fermented milk

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