Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

Takumi Abe; Norifumi Miyake; Yasuyuki Nishijima; Ryousuke Fujita; Ken Sahara; Shin Ichiro Asano; Hisanori Bando

doi:10.1016/j.virusres.2005.03.019

Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

Takumi Abe, Norifumi Miyake, Yasuyuki Nishijima, Ryousuke Fujita, Ken Sahara, Shin Ichiro Asano, Hisanori Bando

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.

Original language	English
Pages (from-to)	38-41
Number of pages	4
Journal	Virus Research
Volume	112
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virusres.2005.03.019
Publication status	Published - Sept 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Cancer Research
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2005.03.019

Cite this

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title = "Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus",

abstract = "We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.",

author = "Takumi Abe and Norifumi Miyake and Yasuyuki Nishijima and Ryousuke Fujita and Ken Sahara and Asano, {Shin Ichiro} and Hisanori Bando",

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month = sep,

doi = "10.1016/j.virusres.2005.03.019",

language = "English",

volume = "112",

pages = "38--41",

journal = "Virus Research",

issn = "0168-1702",

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number = "1-2",

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TY - JOUR

T1 - Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

AU - Abe, Takumi

AU - Miyake, Norifumi

AU - Nishijima, Yasuyuki

AU - Fujita, Ryousuke

AU - Sahara, Ken

AU - Asano, Shin Ichiro

AU - Bando, Hisanori

PY - 2005/9

Y1 - 2005/9

N2 - We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.

AB - We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.

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Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

Abstract

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