Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

Takumi Abe, Norifumi Miyake, Yasuyuki Nishijima, Ryousuke Fujita, Ken Sahara, Shin Ichiro Asano, Hisanori Bando

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5 Citations (Scopus)


We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.

Original languageEnglish
Pages (from-to)38-41
Number of pages4
JournalVirus Research
Issue number1-2
Publication statusPublished - Sept 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Virology
  • Infectious Diseases


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