TY - JOUR
T1 - Enhancement of anti-tumor activity of gamma-irradiated silk fibroin via immunomodulatory effects
AU - Byun, Eui Baek
AU - Sung, Nak Yun
AU - Kim, Jae Hun
AU - Choi, Jong il
AU - Matsui, Toshiro
AU - Byun, Myung Woo
AU - Lee, Ju Woon
N1 - Funding Information:
This research was supported by the National Research Foundation of Korea; Ministry of Education, Science and Technology; and the Korean government through its National Nuclear Technology Program and the Basic Research Program of KAERI.
PY - 2010/6
Y1 - 2010/6
N2 - Silk fibers have proven to be effective in many clinical applications as well as for clothing. In addition to the substantial effect of silk fibers, the present study was conducted to explore its importance in a new dimension to reinforce the effects of its physiological function regarding anti-tumor activity and immune response with gamma-irradiated silk fibroin (GISF). The cytotoxicity results showed that pre-treatment of GISF in the mouse peritoneal macrophages (MPM) indicated a higher proliferative effect than that of non-irradiated silk fibroin (NISF) in a concentration-dependent manner. Based on the cytotoxicity result of MPM, GISF (50 and 150. kGy) was selected for an ex vivo study in an animal (C57BL6) system and evaluated about whether the non-specific immune response was also related to GISF. GISF (50 and 150. kGy) augmented immune responsiveness via activation of NK cells, T lymphocytes proliferation, NO production, and cytokine level, such as IL-6, IL-2, IL-12, IFN-γ, TNF-α, as compared with NISF, which strongly suggested that GISF significantly augmented an important element of all aspects of the innate and adaptive immune system. Therefore, from these results, it seems likely that the GISF will play a potent role in eliciting the effect of the non-specific immune response and anti-tumor activity as a value-added product in the medical industry.
AB - Silk fibers have proven to be effective in many clinical applications as well as for clothing. In addition to the substantial effect of silk fibers, the present study was conducted to explore its importance in a new dimension to reinforce the effects of its physiological function regarding anti-tumor activity and immune response with gamma-irradiated silk fibroin (GISF). The cytotoxicity results showed that pre-treatment of GISF in the mouse peritoneal macrophages (MPM) indicated a higher proliferative effect than that of non-irradiated silk fibroin (NISF) in a concentration-dependent manner. Based on the cytotoxicity result of MPM, GISF (50 and 150. kGy) was selected for an ex vivo study in an animal (C57BL6) system and evaluated about whether the non-specific immune response was also related to GISF. GISF (50 and 150. kGy) augmented immune responsiveness via activation of NK cells, T lymphocytes proliferation, NO production, and cytokine level, such as IL-6, IL-2, IL-12, IFN-γ, TNF-α, as compared with NISF, which strongly suggested that GISF significantly augmented an important element of all aspects of the innate and adaptive immune system. Therefore, from these results, it seems likely that the GISF will play a potent role in eliciting the effect of the non-specific immune response and anti-tumor activity as a value-added product in the medical industry.
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U2 - 10.1016/j.cbi.2010.03.032
DO - 10.1016/j.cbi.2010.03.032
M3 - Article
C2 - 20338156
AN - SCOPUS:77952955094
SN - 0009-2797
VL - 186
SP - 90
EP - 95
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
IS - 1
ER -