TY - JOUR
T1 - Enhanced dynamic instability of microtubules in a ROS free inert environment
AU - Islam, Md Sirajul
AU - Kabir, Arif Md Rashedul
AU - Inoue, Daisuke
AU - Sada, Kazuki
AU - Kakugo, Akira
N1 - Funding Information:
We would like to thank Professor Takashi Hashimoto, Graduate School of Biological Science, Nara Institute of Science and Technology, Japan for fruitful discussions. This research was financially supported by a Grant-in-Aid for Scientific Research on Innovative Areas under “The Plant Cell Wall as Information Processing System” (Grant Number 25114501 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan .
Publisher Copyright:
© 2015 Published by Elsevier B.V.
PY - 2016/4
Y1 - 2016/4
N2 - Reactive oxygen species (ROS), one of the regulators in various biological processes, have recently been suspected to modulate microtubule (MT) dynamics in cells. However due to complicated cellular environment and unavailability of any in vitro investigation, no detail is understood yet. Here, by performing simple in vitro investigations, we have unveiled the effect of ROS on MT dynamics. By studying dynamic instability of MTs in a ROS free environment and comparing with that in the presence of ROS, we disclosed that MTs showed enhanced dynamics in the ROS free environment. All the parameters that define dynamic instability of MTs e.g., growth and shrinkage rates, rescue and catastrophe frequencies were significantly affected by the presence of ROS. This work clearly reveals the role of ROS in modulating MT dynamics in vitro, and would be a great help in understanding the role of ROS in regulation of MT dynamics in cells.
AB - Reactive oxygen species (ROS), one of the regulators in various biological processes, have recently been suspected to modulate microtubule (MT) dynamics in cells. However due to complicated cellular environment and unavailability of any in vitro investigation, no detail is understood yet. Here, by performing simple in vitro investigations, we have unveiled the effect of ROS on MT dynamics. By studying dynamic instability of MTs in a ROS free environment and comparing with that in the presence of ROS, we disclosed that MTs showed enhanced dynamics in the ROS free environment. All the parameters that define dynamic instability of MTs e.g., growth and shrinkage rates, rescue and catastrophe frequencies were significantly affected by the presence of ROS. This work clearly reveals the role of ROS in modulating MT dynamics in vitro, and would be a great help in understanding the role of ROS in regulation of MT dynamics in cells.
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U2 - 10.1016/j.bpc.2015.11.003
DO - 10.1016/j.bpc.2015.11.003
M3 - Article
C2 - 26774598
AN - SCOPUS:84953743278
SN - 0301-4622
VL - 211
SP - 1
EP - 8
JO - Biophysical Chemistry
JF - Biophysical Chemistry
ER -