Enhanced cell-seeding into 3D porous scaffolds by use of magnetite nanoparticles

Kazunori Shimizu, Akira Ito, Hiroyuki Honda

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)


To engineer functional tissues, a large number of cells must be successfully seeded into scaffolds. We previously proposed a methodology for tissue engineering using magnetite nanoparticles and magnetic force, which we termed "Mag-TE." In the present study, we applied the Mag-TE technique to a cell seeding process and have termed the technique "Mag-seeding. " The cell-seeding efficiency of NIH/3T3 fibroblasts (FBs) by Mag-seeding was investigated using six types of commercially available scaffolds (5 collagen sponges and 1 D,D-L,L polylactic acid sponge) having various pore sizes. FBs were magnetically labeled with our original magnetite cationic liposomes (MCLs), which have a positive surface charge, to improve adsorption onto the cell surface. FBs labeled with MCLs were seeded onto a scaffold, and a magnet (4 kG) was placed under the scaffold. Mag-seeding facilitated successful cell seeding into the deep internal space of the scaffolds. Cell-seeding efficiency increased significantly in all scaffolds when compared to those without magnetic force. Moreover, when a high-intensity magnet (10 kG) was used, cell-seeding efficiency was significantly enhanced. These results suggest that Mag-seeding is a promising approach for tissue engineering.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number2
Publication statusPublished - May 2006

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biomaterials


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